Primers used for PCR amplification of the m.3243A>G and m.8993T>G mutations and the m.8993T>G digestion control amplicon
| Primer name | Primer sequence | (C) pmol/µl primer used |
|---|---|---|
| NARP_294F | CACACCTACACCCCTTATCCC | 0.2 |
| NARP_229R | TCATTATGTGTTGTCGTGCAG | 0.2 |
| NARP229R-VIC | TCATTATGTGTTGTCGTGCAG | 0.3 |
| HpaIINARP (6-FAM) | CCTCTACCTGCACGACAACAC | 0.4 |
| HpaIINARP-R | GAGGAGCGTTATGGAGTGGA | 0.4 |
| MELAS_200R | TTTCGTTCGGTAAGCATTAG | 0.3 |
| MELAS_82F | CAACTTAGTATTATACCCACAC | 0.06 |
| MELAS_82F-6FAM | CAACTTAGTATTATACCCACAC | 0.3 |
C; concentration MELAS, mitochondrial encephalopathy, lactic acidosis and stroke-like episodes; NARP, neuropathy, ataxia, and retinitis pigmentosa.