The primer sequences and PCR conditions used for amplification for regulating region, exons and intron–exon junction of the hURAT1 gene
| Amplicon | Primer sequences(forward, reverse) | Annealing temperature, °C | Size of PCR product (bp) |
| Promotor-1 | ACA CTG TGA CAT GAG AGG CCA TAGC TTG TGC AAG TCT CTG TGT GG | 62 | 304 |
| Promotor-2 | CCG CCA GGA GAA AAA CTT AGG CCCCA TCG TCT GGA GAA CCT GGA AC | 64 | 586 |
| Exon 1 | CAT GGC ATT TTC TGA ACT CCT GTTT GAG TCT GAC CCA CCG TG | 62 | 479 |
| Exon 2 | CTC CTC AAA AGG CCA AAC TAT GCCCCT ACA TTC TCC ACC TAC GGA ACC | 62 | 678 |
| Exon 3+4 | GTT TGT GCC GCA GGT TTG GTGT CTT GGG TTT GTG GGT GG | 62 | 583 |
| Exon 5+6 | ACT TAA GGT GGC TGG CAG AGTGG ATC ACC CCT TCG TCA T | 60 | 698 |
| Exon 7 | GCC CCC ACC GCC CAT TGT TCCCTC CGG CAG CCT CCT TCC TAC CAG | 64 | 425 |
| Exon 8+9 | TGA GCG TGG AGC AGA TTG TGGTG TGA CTC TGG GCC AAT CC | 62 | 893 |
| Exon 10 | CTG GGC CCC CGA GAG CAGGG GCC CCT TGG ACT GTG AG | 66 | 362 |
hURAT1, human urate transporter 1.