Table 3 Summary of MS-MLPA methylation indices in 75 control samples and normal and abnormal ranges for these values

	H19 DMR	KvDMR1
Control samples
Mean	0.50	0.53
Range	0.45–0.55	0.47–0.60
Standard deviation	0.03	0.03
Normal and abnormal ranges
Hypermethylation	>0.59
Borderline hypermethylation	0.56–0.59
Normal	0.44–0.56	>0.47
Borderline loss of methylation	0.41–0.44	0.44–0.47
Loss of methylation	<0.41	<0.44

Where repeat analysis was performed, the mean value is given. The H19 DMR methylation index was calculated from the mean value of indices of probes 6266-L5772 and ICR1. The KvDMR1 methylation index was calculated from the mean value of indices of probes 7171-L6780, 6276-L5782, 7172-L6781 and 7173-L6782.