Table 1

 PCR primer pairs for DNA sequencing of the Sp110 gene

LocalisationForward primerReverse primerSize (bp)°C/MgCl2
5′Exon3′
*5′-UTR1/2 and 5′-UTR3/4 are primer pairs to amplify 1092 bp of the 5′-UTR proximal to the ATG; †primers for exons 15a and 15b are designed to amplify the alternative transcripts Sp110a and Sp110b, respectively.
AC009950.6 is the reference sequence for the design of all sequencing reactions and subsequent alignments. All primers anneal to 5′- and 3′ intronic regions of exons. Sequencing of exon 18 comprises 116 bp of the 3′-UTR.
5′-UTR3/4*TCCCTGTAAACAGTTGTTAATTACGGTTGCACAACTAGAGAGTGGAAGAG54562/1.5
5′-UTR1/2*CTTGTTTAACCCAGATCAGTGTGGGTTGCAGTAAGCCAAGATTGTG54762/1.0
Exon 2CAGAGAGAAGCTTCCCACATTCTATGACACCCTCTCTCCTAACTAC35614713962/1.5
Exon 3GAATTTCTTCTTAAAAGAGTAAGGTAGCGATTAACGTGCATATTCCACAGG861697158/1.5
Exon 4ATCCTATTGGAAGGCATGTATTAAGATAAAAGTCAAGATGCTGGGATTG662676658/1.5
Exon 5TTGTGCCTTGATGCTCTATCTGTCTGTTTCTTTTCCTTTGTATTGC798410762/1.5
Exon 6GTTGCTTACTTTCCCACTCTATGCCTCTCTAGAAGATCCGAATGGC131846162/1.5
Exon 7CTGGGCTGCATGTACTTTCTACAGGACTGTGGTCACATAGTGGTG84788358/1.5
Exon 8CTAAGGTCACCAATGAACTTCTAGCTAGAATAAAATTCAACCCTCCACAG99696562/1.5
Exon 9GAGAAGGGAAGTCACTGATTGGGTTATACCCCATCCTCATTCTCTG6215011258/1.5
Exon 10ATGCATTGTTAGGAGGGTAAGACCGTTAAGAAATATTGCCTCAGTGTAAG109816158/1.5
Exon 11ATGGCAAGATAACTCCTGAAAGGGTACGTCTCCCTCCTTCTACTATTG611508862/1.5
Exon 12TGCTACAAGGAAGTCCTTTCATAAGGAGAACAACTTTGGAGGAGAAGG125699258/1.5
Exon 13GTACATTATTGGAATTCATGGAGGGGTCCATTTCCTGATCAATTACACAC64997262/1.5
Exon 14GTAGAAGGCCACAATTAGGATGAAGTCCTTATAAATCATGCTGTTGAATC601439262/1.5
Exon 15a†CAGACTCTCTGCTTGTTGTGCCTCCAGGGAATAGCATACTAGGAAAG16911618958/1.5
Exon 15b†ATCTAGAGAGGGAGAAATGGAAGGATCCTCCTACCTCAGCCTTAGC26325116262/1.5
Exon 16TTGTCACACATTTCACCATAGTCTCCCAGTCAGCATTCCAGCCATGC34310918058/1.5
Exon 17TATCATTATGACCCTGGAAATGAAGGGCTGAAACTGAGTGTAATACTTGC791417162/1.5
Exon 18ATGTGCCTGTAGTTTCAGCTTCTCGTGATAATCCTATGAAGTGTCTGGG19511411658/1.0