Primers used for allele-specific polymerase chain reaction
| Primer location | Primer sequence |
|---|---|
| 3′UTR sequencing primers | |
| UTR-F | 5′-gcaaggagagcagccccaagg-3′ |
| UTR-R | 5′-agttctcaacactgtcacatc-3′ |
| 3′UTR allele-specific primers(+878C→G; use with EX4-F or IVS3-2F primer) | |
| 878C | 5′-ccctgtccactaagtcacag-3′ |
| 878G | 5′-ccctgtccactaagtcacac-3′ |
| Intron 3 sequencing primers | |
| IVS3-F | 5′-aagttctcaaggtagcag-3′ |
| IVS3-R | 5′-aggaacaattagaggctc-3′ |
| Intron 3 allele-specific primers (+266C→T) | |
| 266C | 5′-gcagaggaacttgcagagcc-3′ |
| 266T | 5′-gcagaggaacttgcagagct-3′ |
| Intron 3 allele-specific primers (+516C→T) | |
| 516C | 5′-atgggcctcagcctgacaac-3′ |
| 516T | 5′-atgggcctcagcctgacaat-3′ |
| Intron 3 allele-specific primers (+648A→G) | |
| 648A | 5′-gcagtgtgactctcgttcaa-3′ |
| 648G | 5′-gcagtgtgactctcgttcag-3′ |
| Common reverse primer for intron 3 ASP | |
| EX4-R | 5′-tgagtcttagctggctccttg-3′ |
| Extra sequencing primers | |
| IVS3-2F | 5′-gagcctctaattgttccttg-3′ |
| EX4-F | 5′-tccacccaggtcatggtgatc-3′ |