Table 1

 Amplicons, primers, and probes

GeneForward primer (5′to 3′)Reverse primer (5′ to3′)TaqMan probe*
Primers were designed for two or three distinct loci from each target gene. Primer pairs for each amplicon, associated with SYBR Green or a TaqMan probe, are shown. SYBR Green associated primers were designed in the laboratory with Primer Express software; primers and their associated TaqMan probes were designed using the Primer by Design services from Applied Biosystems. *FAM-5′ to 3′-TAMRA.
SIX3cccaaccaccatctagcactaccatcagcgtttgactgtctgttgaccccaaaggacccc
SIX3tcctgagcaaaaaggcaaagatctgaatgcatactgtggtgaagtgSYBR Green
SHHtctatccagggccctaactgtaggccaatttgtcacgtccccttaaatcgccccaccctctc
SHHcagtcaaggaacacagcaaagagattggtgagatggtcgatgcatSYBR Green
ZIC2tcagacggatgttttcagtctcaggacgcagaacagcacagagtSYBR Green
ZIC2cgaaagagagaatgggtcaaagagcacatgcagagacctcacttcSYBR Green
TGIFggaggactgacaggtctagagacacagagcccctcacttattcccacgcgctgtctgttg
TGIFtgggatcagagcgtcctgttcagccgactctcccgtaactSYBR Green
TGIFccggacctcaaccaggactttctctgcagcccgtttgagtSYBR Green
DISP1atttgggccagtgacctcaatgctctttgatgtcccctttcccctgctccctaaattt
DISP1gactttccaatcgactgaggtatcagtttgtggctttggtctatgtgaaaactgacagaaaatcc
FOXA2gcctgaagccgtcgtcttccgcagatacctcctactaccatcccggcccattatg
FOXA2caccttcaggaaacagtcgttgagcagcgctggcagaacccatccgccactcgct
GAPDHctccccacacacatgcacttaccctagtcccagggctttgattSYBR Green