Primer sets used for genomic sequences
| Patient | Primer orientation | Primer sequence (5′ to 3′) | Primer location | Annealing temperature (°C) |
|---|---|---|---|---|
| A, B | Sense | CGTGGAGCGCATCGACGAGCCC | Exon14 (1155–76) | |
| and their family members | Antisense | CCGCGAAAGCGCTGCAGGACC | Exon14 (1314–34) | 62 |
| E | Sense | CCAGTGCCTCCTGAAAGTCACTG | IVS7 | |
| Antisense | CTCTCGCCACACAGCAACACTGC | IVS8 | 58 | |
| F | Sense | AGGTCCTGCAGCGCTTTCGCG | Exon14 (1313–33) | |
| Antisense | CGCAGGTCAGCAGCTCTATGC | Exon15 (1382–02) | 55 |