Exon | Primer sequence (5′→3′) | PCR product size (bp) | MgCl2, (mmol/l) | Temp (°C) | ||||
---|---|---|---|---|---|---|---|---|
F, Forward; R, Reverse. | ||||||||
1F | CCACCAACTTCCGCCCAAGCC | 372 | 1.5 | 60 | ||||
1R | CCGGCTCCCAGCCTCTGCAGC | |||||||
2F | CCTGCCACCAATACACACGC | 389 | 1.5 | 60 | ||||
2R | TGTCCCTGTTTCTCCATATCC | |||||||
3F | TGCATCTAGCTGCCAAGGCC | 375 | 1.5 | 67 | ||||
3R | TTATTCATGCACAGGCAGCC | |||||||
4F | ATCAGCATGTCACTAACCACC | 320 | 1.5 | 57 | ||||
4R | AACATAATCCAGGAGATAACC | |||||||
5F | AGCATTACCTAGCCTTTCTCC | 299 | 1.5 | 58 | ||||
5R | CATCTGGAAGGCCAGAGTTTTCC | |||||||
6F | AGACCAATTTCCCTCTATGTCCC | 368 | 1.5 | 59 | ||||
6R | AGCATCCTTCAACTTCAGCTTCC | |||||||
7F | GGTGCAGAACTGATGCAGGG | 402 | 1.5 | 60 | ||||
7R | GAAAGCTTCAAGAGAACCGGAG | |||||||
8F | GAGGAAGCCTGTGTGTCCTGG | 283 | 1.5 | 62 | ||||
8R | TATTGGTTCTCCTCCAGTTAG | |||||||
9F | GGGTGGCTAGAGTCTGCAAGG | 623 | 1.5 | 60 | ||||
9R | GAAAGTGCTGAGTAGGTGCTGG | |||||||
10F | CTCCCTTGGCCCTTATCAACAC | 409 | 1.5 | 60 | ||||
10R | GGCATAGTAAATGCCCAATAAA | |||||||
11F | CCACCCTGAAATGAGGACTGCC | 434 | 1.5 | 65 | ||||
11R | CCAGGCACATAGCAGGTGCTC | |||||||
12F | CCGCGCCCAGTACATATCACCC | 335 | 1.5 | 68 | ||||
12R | CCCAGCACGCTGGTGCAGGCC | |||||||
13F | CCTGACCATCAAATGGCCTTCC | 441 | 1.5 | 60 | ||||
13R | CCATCCCTGGAGGAACCCACTGC | |||||||
14F | TTCCGCGTTCCTCTCTCTGTTCC | 361 | 1.5 | 55 | ||||
14R | TCGGGTGTTCAGATCTCAAGCC | |||||||
15.1F | GGGCTGGGTCCAAAGATAGG | 437 | 1.5 | 71 | ||||
15.1R | GACTGCTGGGCAGCCAGGGCG | |||||||
15.2F | CTTTTCATACCCTGTGATCTCC | 531 | 2.5 | 65 | ||||
15.2R | CAGAGTAGCAGAAGCTCCTCTC |