Assay details used for expression studies
| Gene(s) | Primer sequences and labels | Restriction enzyme | Product sizes in bp (Y allele underlined) |
|---|---|---|---|
| PGPL | A: aatggtcgtgtccaccaaaac TET, B: ggcttccagttctttcttggtc | AatII | 158>158+95 |
| DXYS155E | A: ttcagtttcaaacacgtagtcctt FAM, B: cgccaagcagacacagaaat | NlaIII | 155>155+130 |
| SLC25A6 | A: agcacgccagcaagcagat, B: acagcacgccctgctcctt TET | BslI | 91>91+61 |
| MIC2 | A: tcaggtggagaaggaaaagg, B: tgaagcatagcttctttttctgg FAM | HaeIII | 168>168+103 |
| RPS4X/Y | A: ccctgctggattcatggatgt FAM, B: ctcactttgcacaacttgtactt | HaeIII | 135>110+75 |
| ZFX/Y | A: catgatagtgtagtggaagcagaaa TET, B: atcttctccagggtcagcttta | BamHI | 318>249+117 |
| PRKX/Y | A: gtctgtcctgaaggaagtcagc HEX, B: tccaagtcctgtctaccagctt | PstI | 310>310+170 |