Gene variants, primers and restriction enzymes
Variant | Primers 5′–3′ | Enzyme | ||
---|---|---|---|---|
*Genotyped using a reverse tailed restriction site removing PCR (tail is in italic, mismatched nucleotides are underlined). | ||||
Ala203Pro | GTGGGGCTTTGTCAGTGAAT | NlaIV | ||
GGACTCCTGGAGGCATGGTG | ||||
Arg265Gln | GGGCCAGCCAGTGACCATGC | BsoBI | ||
CAGGCGTGGCCAGACGGTA | ||||
Val279Ile* | GCAGGGCAGACCCTGGTGC | HpaII | ||
CAGGAAACAGCTAGTACCGCCGGGACCAGGGCCAGG | ||||
Arg292Ser | CCGAGCCCCCAGCCAGCT | AluI | ||
CAGGCGTGGCCAGACGGTA |