Primer sequences, primer spanning regions, amplified regions, and temperatures of denaturating high performance liquid chromatography analysis
| Primer sequences | Primer spanning region | Regions | Temp for DHPLC analysis (°C) |
|---|---|---|---|
| Primer spanning region relates to DNA sequence numbering, with +1 as the first transcribed nucleotide. Gene bank accession numbers were AF404773 for IL12A (p35) and AY008847 for IL12B (p40). | |||
| DHPLC, denaturating high performance liquid chromatography analysis. | |||
| IL12A(p35) | |||
| 5′tccccactactctcagcaca3′ | −941/−922 | Promoter | 63; 64 |
| 5′cgataggatgaatacacagagtcc3′ | −598/−621 | ||
| 5′ggagcctttgctgactcct3′ | −693/−675 | Promoter | 59.6 |
| 5′ggacacccagggagaacag3′ | −451/−469 | ||
| 5′tgtgaacaaaacgccttgag3′ | −520/−501 | Promoter | 61.8 |
| 5′gccccgtgtctaagctacc3′ | −241/−259 | ||
| 5′gggaggcactcctctctcta3′ | −313/−294 | Promoter | 65.5; 67 |
| 5′cggcgctttcggattaact3′ | −71/−89 | ||
| 5′actgcgaacatttcgctttc3′ | −171/−152 | Promoter | 69.3 |
| 5′acatcagcttctcggtgaca3′ | −22/−41 | ||
| 5′cgggagttaatccgaaagc3′ | −93/−75 | Promoter and exon 1 | 63.8 |
| 5′cgctggatgcagacctgt3′ | +129/+112 | ||
| 5′agtcccgggaaagtcctg3′ | +19/+36 | Exon 1 | 65.0; 67.0 |
| 5′gagcgagccaaagacctg3′ | +213/+196 | ||
| 5′atccaggctgaagctcctc3′ | +1130/+1148 | Exon 2 | 63.2 |
| 5′gagggtgcaggtccacag3′ | +1357/+1340 | ||
| 5′ggtgccaggtgcattatcaa3′ | +3961/+3980 | Exon 3 | 56.9; 57.9 |
| 5′ttttaaccacactgcatgctc3′ | +4171/+4151 | ||
| 5′ttgagacctgaagagtcagaaaga3′ | +4385/+4408 | Exon 4 and exon 5 | 56.2; 57.8 |
| 5′cccattgcatttgaggaaag3′ | +4648/+4629 | ||
| 5′tggatacttccccatcttgtc3′ | +4673/+4693 | Exon 6 | 58.7 |
| 5′gcatgaaaaaggtgctctca3′ | +4888/+4869 | ||
| 5′ttggaataccatgtaagtcatgc3′ | +6368/+6390 | Exon 7 and UTR | 56.1; 57.4 |
| 5′tgatcagaggtatcatgtggatg3′ | +6699/+6677 | ||
| 5′gaactagggagggggaaaga3′ | +6642/+6661 | UTR | 55.0 |
| 5′gctcagatgctttcatgattacc3′ | +6924/+6902 | ||
| IL12B (p40) | |||
| 5′ggaaagttagcccggaaatc3′ | −1246/−1227 | Promoter | 66.0; 68.0 |
| 5′accaggtacacgcagacaga3′ | −983/−1002 | ||
| 5′gtctcgcagcgacctctgt3′ | −1045/−1027 | Promoter | 64.6; 65.6 |
| 5′tgctaagaggtatgcaaaggtg3′ | −892/−913 | ||
| 5′ttcctgttcacgtgcagact3′ | −965/−946 | Promoter | 60.7 |
| 5′cggatgggagcagaaagtg3′ | −707/−725 | ||
| 5′gccttgagtgtggttgtctg3′ | −766/−747 | Promoter | 60.3; 63.0 |
| 5′tttaccacccatcttggctc3′ | −521/−540 | ||
| 5′tcccactcggggaatgtg3′ | −572/−555 | Promoter | 59.3 |
| 5′tgagttcatgtcgggatgg3′ | −365/−383 | ||
| 5′ggattttgtttttaagcccttg3′ | −419/−398 | Promoter | 61.2; 63.0 |
| 5′aaggaaggagatggagatgct3′ | −231/−251 | ||
| 5′agtgctgtctccaagcacct3′ | −298/−279 | Promoter and exon 1 | 57.5 |
| 5′agtccaatggccctgaaac3′ | +37/+19 | ||
| 5′tccagtaccagcaacagcag3′ | −19/+1 | Exon 1 | 62.0; 64.0 |
| 5′agggaggggagggaggtag3′ | +96/+78 | ||
| 5′cttctcttccccctgcttct3′ | +3674/+2693 | Exon 2 | 60.0; 61.0 |
| 5′ggggcctccacagagaata3′ | +3830/+3812 | ||
| 5′gcttacagcctggttctcca3′ | +7124/+7143 | Exon 3 | 57.2; 60.5 |
| 5′atgagcgaaagagaaaattgat3′ | +7481/+7460 | ||
| 5′cacaatcaaaacctttctgcaa3′ | +7916/+7937 | Exon 4 | 56.1; 59.7 |
| 5′aaatgctgagaaaccagagca3′ | +8128/+8108 | ||
| 5′ggaactctctccccaatgtg3′ | +9918/+9937 | Exon 5 | 59.0; 62.0 |
| 5′tttccttatggagcacatataatca3′ | +10221/+10197 | ||
| 5′tgaactccaaagccttattaggaa3′ | +11545/+11568 | Exon 6 | 58.4; 60.0; 61.0 |
| 5′gcctccgtaaaactgaccaa3′ | +11804/+11785 | ||
| 5′aggggtcttcacagctttcc3′ | +13642/+13661 | Exon 7 | 60.9; 63.2 |
| 5′ccaatcatatccctgcatcc3′ | +13875/+13856 | ||
| 5′tagcttccatggcagtcctc3′ | +15315/+15334 | UTR | 59.1 |
| 5′gggattccagattttctttgc3′ | +15524/+15504 | ||