Names and sequences of probes used for MLPA for the α-globin gene cluster and flanking regions
| No. | Name | Standard deviation (range)† | Upstream hybridising sequence | Downstream hybridising sequence | Position on chr. 16p13.3‡ |
|---|---|---|---|---|---|
| *With MLPA-F and -R common ends (probes labelled in blue in figures); †calculated on 12 normal individuals; ‡UCSC Genome Browser (May 2004). | |||||
| 1a | c16orf33 | 0.05 (0.93–1.10) | CACAGTCCTGGACCTGAAGAAGGCCATC | CAGAGATACGTGCAGCTCAAGCAGGAGCGT | 45799–45856 |
| 2a | c16orf8/RHBDF1 | 0.05 (0.90–1.08) | CACGAGCAGCCTGCAGCGCAAGAA | GCCACCCTGGCTAAAGCTGGACATTCCCTC | 54937–54990 |
| 3a | MPG | 0.06 (0.84–1.07) | CAAGAGCTTTGACCAGAGGGAC | CTGGCACAGGATGAAGCTGTATGGCT | 75563–75610 |
| 4a | c16orf35 (2)* | 0.04 (0.97–1.14) | CTAACCATGCACACAGATCAGAAAACACC | CTGCTCAATGGTGCTGATTCCCCTGCT | 90548–90603 |
| 5a | c16orf35 (HS-40) | 0.09 (0.86–1.17) | GTGAATGGTACTGCTGATTACAACCTCTGGTGCTGCCTCCCCCTC | CTGTTTATCTGAGAGGGAAGGCCATGCCCAAAGTG | 103695–103774 |
| 6a | c16orf35 (3)* | 0.11 (0.84–1.18) | GTAAGCCGCGTAGCAGATACGCTGC | CAGCAACACGGGCGACCATGCTGAT | 120541–120590 |
| 7a | L1 | 0.07 (0.86–1.12) | CCTGGACAATGAAGCACCAGGGCCAAC | CTCCATTTGCTACAGGGGACATCCT | 132952–133003 |
| 8a | L0 | 0.04 (0.93–1.09) | GTGACCAGGGAGGGCCAGTTCATCTCGGTCTGAAAGAAGC | CCCAGATGAGCAAAGGATACACTGGCCTCCTG | 139157–139228 |
| 9a | HBZ | 0.05 (0.90–1.09) | CAGATCCAGTACATCTCCCCTCAGCGCTGGGTGGACCTAAC | CCTTGCTTTCTTGGAGGAAACCCAGGAATCCAG | 142344–142417 |
| 10a | HBZP | 0.05 (0.89–1.09) | CTCTAGGTCACCCTGTCATCACAGGGACAGGGAG | GTCAAGGACAGTCACTCCTGAGGCCA | 152736–152795 |
| 11a | HBAP2 | 0.09 (0.92–1.21) | GTGCAAATGCAAGCGGCGTGGGACAAGTTC | CTGACTGGTGTGGCCGTGGTGCTGACCGAAAA | 156636–156679 |
| 12a | HBAP1 | 0.04 (0.92–1.07) | GACTCAGAAATAAGCTGCCGTGGTGCTGTCTC | CTGAGGACAAGGCTAACACCAAGGCGGTCTGGGAGA | 158659–158726 |
| 13a | ψα1-α2* | 0.09 (0.87–1.16) | CATCCCATGCTGAGGGAACACAG | CTACATCTACAACTACTGCCACAGGCTCTCT | 160969–161022 |
| 14a | HBA2 | 0.11 (0.84–1.15) | GAAGATCCAACGGGGGAAGCATTG | CTAAGCTGGTCGGAGCTACTTCCTTC | 165128–165176 |
| 15a | HBA1 | 0.04 (0.88–1.03) | GTGCCAAGAACTGGCTGGCTTTCTGCCTG | GGACGTCACTGGTTTCCCAGAGGTCCT | 167825–167880 |
| 16a | HBQ | 0.09 (0.71–1.05) | CTGGACAAGTTCCTGAGCCAC | GTTATCTCGGCGCTGGTTTCCGAGT | 171054–171099 |
| 17a | 3′HVR (tel)* | 0.15 (0.78–1.26) | GATGGCTCTGAGGGTGACGCTGTCTGCTTAAGGC | CCAGGGAAACCCAGGTGCAAACTCACACTC | 175806–175869 |
| 18a | 3′HVR (cen)* | 0.06 (0.89–1.09) | CTCAAGGGTGGCATGTGTACC | CCTGCAGAAACAGAGCGGATGAGGA | 177548–177599 |
| 19a | LUC7L (2)* | 0.06 (0.88–1.08) | GTCTGTTCAGCCTACCTTGGTCTCCATGACAATGACCGTCGC | CTGGCAGACCACTTCGGTGGCAAGTTACACTTGGGG | 189203–189080 |
| 20a | LUC7L | 0.05 (0.91–1.10) | GCTGAATGTGATCGGAGAACTGAGCTCGCCAAGAAGCG | GCTGGCAGAAACACAGGAGGAAATCAGTGCGG | 198092–198161 |
| 21a | c16orf9* | 0.21 (0.81–1.37) | CTTGCGAAGGCACAGATTCCCCGTCCACAGCTCAC | GACCAGATGCACCAGCAGGAGTCCACATCGAGGAC | 239560–239629 |
| 22a | PDIP | 0.08 (0.77–1.11) | GAGCTGGCTGAGGAGTTTGGTGTGACGGAGTAC | CCTACGCTCAAGTTCTTCCGCAATGGGAACCGC | 274507–274572 |
| 23a | AXIN1 | 0.04 (0.92–1.09) | GGATGCACACGAGGAGAACCCTGAGAGCATCCTG | GACGAGCACGTACAGCGTGTGCTGAGGACA | 288064–288127 |
| 24a | AXIN1 (2)* | 0.06 (0.89–1.13) | CTCAGAGCCTCCTCAGCAGAAGTCTGAATACATCACTTACC | CAGAACCAGGTTGCCGGCTTACAGTCATGGT | 321023–321094 |
| 25a | c16orf43* | 0.04 (0.95–1.07) | GGAGAGTCCATTGACAAGCTCTTAATCGCAGCTGCCCTTGCAGGGC | CTGTCCCTGAAGTCCCGAGTAATGGGATTT | 350871–350946 |
| 26a | DECR2 | 0.09 (0.81–1.16) | CTTGTCCTTCAACGCCTTCAAGACCGTGATGGACATCGATAC | CAGCGGCACCTTCAATGTGTCTCGTGTGCTCTATGA | 400278–400355 |
| 27a | KIAA0665 (1)* | 0.06 (0.91–1.08) | GGACTTAACTAAGTACTTGGATCCCAGTG | GGCTCGGCGTGATCAGCTTTGAAGACTTC | 451410–451467 |
| 28a | KIAA0665 (3)* | 0.08 (0.86–1.13) | GAACGGATGCTTCTGTCTCTCTAGCACTGACCCTCTTGCCGCAAAG | CTGCACAGCATCCTCACTGATGAGGCGTTTGAGT | 481111–481190 |
| 29a | KIAA0665 (2)* | 0.08 (0.85–1.14) | CGCAACCTGAAGGAGCAGAACGAGGAGCTGAACG | GGCAGATCATTACCCTCAGCATCCAGGGCGCCAA | 510466–510533 |
| 30a | SOLH* | 0.08 (0.76–1.09) | GTCAAGAAGTTCGTCAGCTGCGACGTCATGCTGGAGCCTGGC | GAGTACGCTGTGGTGTGCTGCGCCTTCAACCA | 542420–542493 |
| 31a | RAB40C* | 0.05 (0.92–1.09) | CAGACAGGTGTGTGAGCGTACATTGCCCTCTCCAG | CTTCACAAACAAATGAGTCATCGAAGTGACC | 583598–583663 |
| 32a | WFIKKN1* | 0.06 (0.85–1.11) | CGAGGACGTGCTCAAGGATGACAAGATGGGC | CTCAAGTTCTTGGGCACCAAGTACCTGGAGG | 623788–623849 |
| 33a | RHBDL1* | 0.07 (0.86–1.10) | GATGAGCCAGGCCTAGGTGTCTACAAG | CGGTTTGTGCGTTACGTGGCCTACGAGATCCTG | 666751–666810 |
| 34a | c16orf25* | 0.05 (0.89–1.09) | GTGCTGGTCCACGTACTCCTGTAG | CTCAGAAAGTTGCTCTTCAGCCATCGTG | 712920–712982 |
| 35a | MSLN (ATR-16) | 0.07 (0.85–1.14) | CTGAGGACATTCGCAAGTGGAATGTGACGTCCCTGGAGAC | CCTGAAGGCTTTGCTTGAAGTCAACAAAGGGCACGA | 756403–756478 |