Primers used to amplify MMP20
| Exon | Sequence 5′–3′ | Size (bp) | Annealing temp (°C) |
|---|---|---|---|
| 1 | F: TACCCCATCATCAGGGAGAG | 468 | 53 |
| R: CCAGACACCAATCTAGGTGGA | |||
| 2 | F: CCCAAAACCAATCATCGTTA | 594 | 55 |
| R: GCCTGACGGATGGATGTAAA | |||
| 3 | F: TCCCTTTCATTTGTCTTGTTGTT | 482 | 53 |
| R: ATGGCTTTGCCATGGTCTT | |||
| 4 | F: TTTTTCAATGGGCTGTTACTCA | 485 | 53 |
| R: CAACACAATTTGGGGTGGAT | |||
| 5 | F: CACCAAAGCTAAGTGCCAGA | 468 | 55 |
| R: CAGCTCTTCACAAGAAGGCATAG | |||
| 6 | F: GAAGCTGAGGCTTGCAAAGT | 490 | 55 |
| R: ACCACCCTTCTGCTGCATAG | |||
| 7 | F: AGGCAAGGCAAGAGCAAAG | 414 | 55 |
| R: TAGGGTGGCATTTCATACCC | |||
| 8 | F: ACTTTTGCCTCCAGGGAAGT | 493 | 55 |
| R: TCTGTAAATCGCACCCCAGT | |||
| 9 | F: TGGCACCACTACACTTCAGC | 570 | 60 |
| R: CAAACAAACAAACAAAAACTTTCC | |||
| 10 | F: GACAAGCTAACTGCCACCTGA | 484 | 55 |
| R: TGCAGAGTGCATTGTGTTGA |