Primer sequences and conditions for SnaPshot reactions in the Newcastle cohort
| PCR | SNaPshot reaction | |||||
|---|---|---|---|---|---|---|
| SNP | Primer sequence (5′→3′) | Annealing temp (°C) | Primer sequence (5′→3′) | Pooled primer conc (µM) | Length (bp) | |
| Forward | Reverse | |||||
| CFH, complement factor H; conc, concentration; MCP, membrane cofactor protein; PCR, polymerase chain reaction; SNP, single nucleotide polymorphism; temp, temperature. | ||||||
| CFH c. −257 C→T | TCTTTACCTTCTCAATATCCAGC | ACTCCTGTGAAAAGCATCATTAG | 60 | (6A)GGGTTTATGAAATCCAGAGGATAT | 0.3 | 30 |
| CFH c.2089 A→G | TATATTGTAAAACAGACAATTTAACC | ATACAAAATACAAAAGTTTTGACAAG | 57 | CTAATGAAGGGACCTAATAAAATTCA | 0.67 | 26 |
| CFH c.2881 G→T | TAAATTTATGAGTTAGTGAAACCTG | TGGTACCACTTACACTTTGAATG | 57 | TTCCTTGTAAATCTCCACCTGA | 0.04 | 22 |
| MCP c. IVS12+638 A→G | CCCAATTTGGTGTCTTTTCA | TCTGTGTTCCAGGATTCATTC | 54 | (19A)GCTGAGAGGGGTTAGATCT | 0.1 | 38 |
| MCP c.2232 T→C | TAATTTCAGAATCAGATGCATCC | TCAAAACCACCTACTTTTAGAGG | 59 | (13A)CGGAGTTCTCTTTGGGAAAAC | 0.2 | 34 |