Table 1

Primer sequences and conditions for PCR reaction and DHPLC analysis of CDKL5 amplicons

Exon	Forward primer	Reverse primer	Product length (bp)	PCR annealing temp (°C)	DHPLC analysis
Exon	Forward primer	Reverse primer	Product length (bp)	PCR annealing temp (°C)	Temp of elution (°C)	Buffer (%)
2	AGGTAAGATTGGTTACTAGAG	AATAACTAACTGTTCATTGCTC	350	58	55	57
2	AGGTAAGATTGGTTACTAGAG	AATAACTAACTGTTCATTGCTC	350	58	57	57
3	TGAGAAGCAATGTCAGTATAG	CCTGTACATGCCCACACGC	201	58	55	53
4	CTGGCTTCTTGCTACTCTG	TCCCACTTCTTCCACACTC	242	58	54	53
5	AGTGTTCTTGGAATTCTTTG	GGCAAATGTGCACATTGGC	244	54	57	53
6	CTCTGTATTGGATGAATTATTC	TTCTTAAAGACAGTAACATGTG	303	59	54	55
6	CTCTGTATTGGATGAATTATTC	TTCTTAAAGACAGTAACATGTG	303	59	56	55
7	TTTATCTTGACACTCCAGA	ACTCCTCCAGCAATCAATT	237	56	53	53
8	GCCCATCGGAGAACAGTCATTAC	GCAAATGACAATAGAATCAGCAG	280	55	56	54
8	GCCCATCGGAGAACAGTCATTAC	GCAAATGACAATAGAATCAGCAG	280	55	57	54
9	TTATTCACTTGTGTTCTGATGAT	CAAATACTGCAGTATTGATTCC	410	54	54	58
9	TTATTCACTTGTGTTCTGATGAT	CAAATACTGCAGTATTGATTCC	410	54	56	58
10	TATGAATTTGACTGGGATTGG	CTATGGTCACATGTAGACAC	275	59	57	54
11	TTGATATTCTGCAATGACTGTG	AGCCACCTCCTCCACCTAC	333	62	53	56
11	TTGATATTCTGCAATGACTGTG	AGCCACCTCCTCCACCTAC	333	62	55	56
12_a	TTGTGTGTCAGCTATTGAGG	GGTTCTGCTGAGATCTGCTG	406	60	56	60
12_a	TTGTGTGTCAGCTATTGAGG	GGTTCTGCTGAGATCTGCTG	406	60	59	60
12_b	CAACAACATACCACACCTTC	TTCTCGTGTCACTGTGTCTG	422	60	57	60
12_b	CAACAACATACCACACCTTC	TTCTCGTGTCACTGTGTCTG	422	60	60	60
12_c	ACTCCAAGTCTGTGAGCAAC	AGATGGACCCTCATCACATC	541	60	57	63
13	GGTTATGGTCCTAGTTCTAC	CACTTCAACTTATTTGTGGG	298	60	57	55
13	GGTTATGGTCCTAGTTCTAC	CACTTCAACTTATTTGTGGG	298	60	59	55
14	CAATAGAGTGAGACCCTGTC	CTGAGTCGGTGAAAGCAGTG	279	65	57	55
14	CAATAGAGTGAGACCCTGTC	CTGAGTCGGTGAAAGCAGTG	279	65	60	55
15	AAAAGTCCATCAGTGACTTAC	CCTAGCAGGAGAAAGGACAC	262	60	56	54
16	TATAGGAACCTAGTGTCATGC	CAACTTTGATTGCCAAGTGC	293	59	53	57
17	CTTGGGTGTGGTTGCATATC	CTGTAACATTGAGAGGCTAAG	296	60	59	55
18	CTTGCACATGCTTGCCCTTC	CACCCAGCTGTTCAGAGTAG	418	62	61	58
19	ACTCTGGTCAATGGGATGTG	CATTCAGTAGTCTAGGGTCG	249	60	59	53
20	TTGGCTTCAGCTGGTGTCTG	CATCTGCATTTCTACAGCTC	345	61	61	58
21	CATTAGCCAGAGTGCACCTG	AGGAAAAACTCAACCTCAGCG	290	60	59	55
21	CATTAGCCAGAGTGCACCTG	AGGAAAAACTCAACCTCAGCG	290	60	63	54