Primers used to screen MLH1 and MSH2 cDNA for mutations and splice changes
| Primer name | Direction | Sequence | Location | Fragment length |
|---|---|---|---|---|
| *From 13 | ||||
| MLH1 | ||||
| RT-Lf1* | Forward | 5′-CATCTAGACGTTTCCTTGGCTCTTC-3′ | 5′ UTR | }1093 bp |
| MLH1-12R | Reverse | 5′-GCAAGTCCTGGTAGCAAAGT-3′ | Exon 12 | |
| MLH1-BF | Forward | 5′-AAGGGACCCAGATCACGG-3′ | Exon 5 | }536 bp |
| MLH1-BR | Reverse | 5′-CTCCAGGATGCTCTCCTC-3′ | Exon 11 | |
| MLH1-CF | Forward | 5′-CGGGTGCAGCAGCACATCG-3′ | Exon 11 | }1446 bp |
| RT-Lr1* | Reverse | 5′-TAAAGGAATACTATCAGAAGGCAAGTATA-3′ | 3′ UTR | |
| MSH2 | ||||
| RT-Sf1* | Forward | 5′-GGCGGGAAACAGCTTAGTGGGTGTG-3′ | 5′ UTR | }1504 bp |
| MSH2-CR | Reverse | 5′-TGAGATTAGGATCAAATGAAGG-3′ | Exon 9 | |
| Sf3* | Forward | 5′GCAGAATTGAGGCAGACTTT-3′ | Exon 7 | }1282 bp |
| MSH2-ER | Reverse | 5′-CAGTTGGTATCTGATTGGC-3′ | Exon 14 | |
| MSH2-EF | Forward | 5′-TTGGAGAAAGGACAAGGAAG-3′ | Exon 12 | }1084 bp |
| RT-Sr1* | Reverse | 5′-CCCATGGGCACTGACAGTTAACACTATG-3′ | 3′ UTR | |