Factor I primer sequences, PCR annealing temperatures, magnesium concentrations, and sequencing conditions
Exon no | Forward primer sequence | Reverse primer sequence | PCR annealing temp. (°C) | MgCl2 conc. | Sequencing temp. (°C) |
---|---|---|---|---|---|
1 | ggaaacaagttcctattggtc | cttgttcagttcaatgcctct | 60 | 1.5 | 60 |
2 | aacaccatcctcatctgtact | tactggaagacttttcactgg | 60 | 1.5 | 60 |
3 | ctgccgctgacacataataaa | tccaagtgttgggtaaacatg | 60 | 1.5 | 60 |
4 | gtttcttacctcgtacagtag | tattattgcctctgtgactgg | 60 | 1.5 | 60 |
5–6 | atctgataaggatgtcatgcc | ccatctatgttccccttaagat | 60 | 1.5 | 60 |
7 | cagctagacatcctacaattc | tgttcaggctgggttattact | 60 | 1.5 | 60 |
8 | acatgccttggggattttgta | taaccagtgagccaccaata | 60 | 1.5 | 60 |
9–10 | tactaatgattccagcctgtc | gctttatcatctgccacaatc | 60 | 1.5 | 60 |
11 | catgacagcaagcatttacaa | ttatgcttctctctgagtgct | 60 | 1.5 | 60 |
12 | aaacacgctaaggaagagttc | attagaggaagaaacctgagc | 60 | 1.5 | 60 |
13 | ttgttaaatgccatggaggag | gacaggtactatgccaaacat | 60 | 1.5 | 60 |