Factor I primer sequences, PCR annealing temperatures, magnesium concentrations, and sequencing conditions
| Exon no | Forward primer sequence | Reverse primer sequence | PCR annealing temp. (°C) | MgCl2 conc. | Sequencing temp. (°C) |
|---|---|---|---|---|---|
| 1 | ggaaacaagttcctattggtc | cttgttcagttcaatgcctct | 60 | 1.5 | 60 |
| 2 | aacaccatcctcatctgtact | tactggaagacttttcactgg | 60 | 1.5 | 60 |
| 3 | ctgccgctgacacataataaa | tccaagtgttgggtaaacatg | 60 | 1.5 | 60 |
| 4 | gtttcttacctcgtacagtag | tattattgcctctgtgactgg | 60 | 1.5 | 60 |
| 5–6 | atctgataaggatgtcatgcc | ccatctatgttccccttaagat | 60 | 1.5 | 60 |
| 7 | cagctagacatcctacaattc | tgttcaggctgggttattact | 60 | 1.5 | 60 |
| 8 | acatgccttggggattttgta | taaccagtgagccaccaata | 60 | 1.5 | 60 |
| 9–10 | tactaatgattccagcctgtc | gctttatcatctgccacaatc | 60 | 1.5 | 60 |
| 11 | catgacagcaagcatttacaa | ttatgcttctctctgagtgct | 60 | 1.5 | 60 |
| 12 | aaacacgctaaggaagagttc | attagaggaagaaacctgagc | 60 | 1.5 | 60 |
| 13 | ttgttaaatgccatggaggag | gacaggtactatgccaaacat | 60 | 1.5 | 60 |