Table 2

 Primers used for polymerase chain reaction amplification of UGT1A7

†Bold “g” in the primer sequence denotes mutagenesis for more selective amplification of the UGT1A7*3 and UGT1A7*4 alleles.
‡Bold “a” in the primer sequence denotes site directed mutagenesis for introduction of a VspI restriction site into the wild-type allele.
FRET, fluorescence resonance energy transfer.
FRET analysis
All individualsSForward5′-TGC CGA TGC TCG CTG GAC G-3′
AReverse5′-ATC TCT ACA GCC ACA CAT CAA TT-3′
Haplotype analysis
3/4F†Forward5′-GGT GGA CTG GCC TCC TTg CA-3′
RReverse5′-CCA TAG GCA CTG GCT TTC CCT GAT GAC A-3′
RFLP analysis
Codon 129 and 131F1‡Forward5′-AAT TGC AGG AGT TTG aTT AA-3′
F2‡Forward5′-AAT TGC AGG AGT TTG aTT A-3′
R1Reverse5′-TTC AGA GGC TAT TTC TAA GA-3′
Codon 208F3Forward5′-ATG CTC GCT GGA CGG CAC CAT TG-3′
R2Reverse5′-TGC CGT GAC AGG GGT TTG GAG A-3′
Allele specificF4Forward5′-ATT GCA GGA GTT TGT TTA AGG ACA-3′
R1Reverse5′-TTC AGA GGC TAT TTC TAA GA-3′