Hoechst 33342 and ethidium bromide double staining of transfected HEK293T and COS-1 cells*
| Cells | Viable (%) | Apoptotic (%) | Late apoptotic (%) | Necrotic (%) |
|---|---|---|---|---|
| *Percentages are the mean of two experiments. Each experiment consisted of two separate counts of at least one field with a minimum of 100 cell counts. With χ2 statistics, p values of <0.001 and 0.279 were calculated in HEK293T and COS-1 cells, respectively, for the differences between transfections with wildtype DFNA5–GFP and mutant DFNA5–GFP. | ||||
| †Typical transfection efficiencies for these experiments can be deduced from table 1. | ||||
| HEK293T | ||||
| Untransfected cells | 98.44 | 0.78 | 0.33 | 0.45 |
| Mock transfected cells | 86.96 | 0.69 | 0.23 | 12.13 |
| 500 ng GFP | 79.22 | 0.65 | 0.86 | 19.28 |
| 500 ng DFNA5–GFP† | 79.10 | 0.45 | 0.67 | 19.78 |
| 500 ng mutant DFNA5–GFP† | 38.40 | 1.40 | 0.25 | 59.96 |
| COS-1 | ||||
| Untransfected cells | 99.69 | 0.18 | 0.14 | 0.00 |
| Mock transfected cells | 96.90 | 0.84 | 0.18 | 2.09 |
| 1 µg GFP | 97.03 | 1.31 | 0.21 | 1.45 |
| 1 µg DFNA5–GFP† | 98.04 | 0.54 | 0.00 | 1.43 |
| 1 µg mutant DFNA5–GFP† | 93.71 | 0.88 | 0.22 | 5.19 |