Table 1

Cell counts on the basis of the number of dots in quadrant analysis for HEK293T and COS-1 cells*

Cells	Viable (%)			Dead (%)
Cells	GFP^–	GFP⁺	Total	GFP^–	GFP⁺	Total
In total, 10⁴ cells were analysed with CELLQuest software. Numbers are deduced from one representative experiment out of two experiments (dot plots are shown in fig 2B). With χ² statistics, p values of <0.001 and 0.171 were calculated in HEK293T and COS-1 cells, respectively, for the differences between transfections with wildtype DFNA5–GFP and mutant DFNA5*–GFP for the experiment represented in table 1 and fig 2. The second experiment gave p values of <0.001 and 0.027, respectively.
HEK293T
Untransfected cells	87.88	0.00	87.88	12.10	0.02	12.12
Mock transfected cells	72.47	0.13	72.60	26.30	1.10	27.40
500 ng DFNA5–GFP	15.09	53.71	68.80	20.26	10.94	31.20
500 ng mutant DFNA5–GFP	16.92	6.87	23.79	32.59	43.62	76.21
COS-1
Untransfected cells	95.09	0.01	95.10	4.87	0.03	4.90
Mock transfected cells	92.55	0.09	92.64	7.17	0.19	7.36
1 µg DFNA5–GFP	79.16	11.27	90.43	8.63	0.94	9.57
1 µg mutant DFNA5–GFP	84.74	4.56	89.30	9.11	1.59	10.70