Table 3

Primer sequences used in the study and their annealing temperatures

Exon	Forward 5′–3′	Reverse 5′–3′	T_m(°C)
Forward primers have −21M13F tail attached for sequencing: TGTAAAACGACGGCCAGT; reverse primers have M13R tail attached for sequencing: CAGGAAACAGCTATGAC.
1	M13F gtgaaccctcagccaatcag	M13R tgacgacgggagaggaag	63
2	M13F tgttggtttcggtgagcag	M13R ggtgtgggagtgcaatttct	61
3	M13F cgctctttggagaaggaatg	M13R aacggtaccaaggctgagaa	58
4	M13F gctgtctggctaggttggac	M13R ttttccctttctctccttgg	58
5	M13F gaaagggaaaagacccagtg	M13R ggatccagcatgggttgac	58
6	M13F gccccttctcccatgttt	M13R ctttgggcttggacaacact	56
7	M13F gggcagaattggattaagca	M13R tgtccacgggattgagcta	57
8	M13F ctgggctaggccaaaggt	M13R ccatgagcagtggtgacactt	57
9	M13F aatcctttagccccctgaga	M13R aggggacaagggtatgaaca	61
10	M13F ccaaaagcaacagttaagga	M13R caaatgacaaaatgccatga	56
11	M13F agcgcttaagccgttttca	M13R gaggggcaaggaactgaact	60
12	M13F aaggcaatggggattcatta	M13R attgaaaggtggggatctgg	59
13	M13F caattttattctggaatgagctttt	M13R caggaaataaacctcctccattt	55
14	M13F gctgcttctggccttcttta	M13R gctgtttcaaatgcctacctct	55
15	M13F tgaacatagccctgtgtgtatg	M13R tttttgacacaactcctcctg	58
16	M13F agacttcttgccccagatga	M13R aaccaccagcaacgtgattt	63