PCR primer sequences
Exon | Forward (5′–3′) | Reverse (5′–3′) | Product size (bp) |
---|---|---|---|
Sequencing primers are the same as PCR primers except for 2–1 forward, 2–1 reverse, 2–2 reverse, and 7–3 reverse. Sequences (5′–3′) of newly designed sequencing primers for 2–1 forward, 2–1 reverse, 2–2 reverse, and 7–3 reverse are AGCGTGGAAGAAGCAACCAC, GTCTTCTGCCCCCAATAGGT, GAAGGCGTTGGTGGAAAAAC, and TTCTCCTCTGCACATTGCTG, respectively. | |||
1 | AGACCCCAGAGCCTGGACTT | GCAAAGTCAAAAGCAGAGCA | 448 |
2–1 | AACGCCTGGACAAAACAAAG | CCAAGCTCTGATACATGGTC | 652 |
2–2 | CAGTTCCTCCCACGCATATT | CTTCTGCCTTCCCTAGAAAC | 872 |
3 | AAAGGGCATTGTTTCTGTGC | GATGCACACCCTCAAGTTC | 377 |
4 | GGGCAGTGCACACCTTTTAC | GAGAGATGGGCATCAGAAGG | 232 |
5 | GCTTAGGTGTTGCCTTCTCG | TGCCTAACCCGGAAGATATG | 270 |
6 | ACTGTAGCCCTCCGCAGATA | TGAGGCCTGGCTGCAAGTC | 404 |
7–1 | ACATCTGCATAGCAGGGCAC | TGCCATGCCTCAAGCGGAT | 755 |
7–2 | CGAGGATCTGAGAACAAGCG | CGTGTTGCAGAATCTCTGGC | 755 |
7–3 | GCTCAAGCCAGTCTGGCAAG | AGTGGGTCAGTGCCTACACG | 755 |