Table 2

 Primers for BMPR2 mutation scanning by DHPLC

ExonForward primerReverse primer
15′-GCA TGA AAG CTC TGC AGC TA- 3′5′-ATT TCC CTG GAA GGC ATG G- 3′
25′-GTC ATT TGG ATA AGA CAA AG- 3′5′-TTT AAC ATA CTC CCA TGT CC- 3′
35′-TAG CCT ACA CGT ACT CTC AC- 3′5′-CCT GGC TTC AAC CTT GAA TG- 3′
45′-GGG TAC AGC CTT TCT AAA GG- 3′5′-GAT ACT ATT GAG GCT GGG TG- 3′
55′-GCT GCT AAT CTT TCT GCA GC- 3′5′-GAA TGA AGT CAC TGT TCC AG- 3′
65′ –CAG AGA GCT GTA GCA TTC TG- 3′5′-AAG TGA TCC ACC TGC CTT AG- 3′
75′-CGC ATT TTT TCC TCT ATA- 3′5′-TGC ATT TCA TGC TGA ATC TTT C- 3′
85′-GCA GAA AAA TAA TAC TAC TTC TAT A- 3′5′-GAT GTT TTT AAT TAA ATT ATC ATT TC- 3′
95′-AGA ATA TGC TAC GTT CTC TC- 3′5′-ACA CTA GAT AGC AAT GAA CTA AAG G- 3′
105′-GCC TGA AGG GGA TGA AAA A- 3′5′-GCC ATT AGG CAA CTC CAA AA- 3′
115′-CCT CAT GTG GTA AAC TGA AAA GC- 3′5′-TGC ACT TGT ACC AAA CAA AAA TG- 3′
12–15′-AAA TTT GGA GAG ACA GTT TGT CA- 3′5′-TTC ATC TTG GTA TGG CAT CTC- 3′
12–25′-AGC AAG CAC AAG CTC GAA TC- 3′5′-AGT CCT GCT GTC CAG TTG CT- 3′
12–35′-TCT AGC TTG CTT TAC CCA CT- 3′5′-AGC ATG GGA GTT AAC ACT GT- 3′
12–45′-ACC TCA TGT GGT GAC AGT CA- 3′5′-ATT GGA ATT AGT TCG GCC AC- 3′
12–55′-ATT CCA GTC CTG ATG AGC AT- 3′5′-AGT TAT TTA AAT GGC CCC AA- 3′
135′-TTA CAT CCC TTA CCC GTT AT- 3′5′-TTA AAG CAA GTC TTT GTT GC- 3′