Table 2

Exon–intron boundaries, sizes and PCR primer sets for amplifying the GNPTAG gene

ExonIntron: 3′splice acceptorIntron: 5′splice donorSize (kb)
Intron sequences are shown in lowercase letters. Exon sequences are shown in uppercase letters. Numbers in parentheses indicate nucleotides number according to clone LA16-316G12 and PCR primer sets for amplifying the 11 exons of ML IIIC gene.
1 (20037–0088)...TCGGCCGGCGgtgagtggcc52
2 (20173–0230)gctccccgtagGGCCCGCGCC......ACGCGTTTGGgtgagcagcc58
3 (20310–0377)cttcccgtagGGTGAACAAC......CCCCTGTCTGgtgagtgagg68
4 (29814–9868)tctcttccagGACCCGTGCA......TGGAGTCCACgtgagtgcag55
5 (29943–0026)gtgtccccagGTACAAGTAT......GGATCCTCGGgtgagtgggg83
6 (30109–0202)gtctccccagCATCTGGCAC......GCAGAGCAAGgtggggcctc94
7 (30277–0391)tccct ccccagGTGGAGCTGG......GCCTTGCTAGgtaggggtgc115
8 (30523–0605)gggtcctcagTGTACCCAAC......CACCCCCCAGgtaagcgtgc83
9 (30682–0813)tccaccttcagGGCCATGAGA......CTGCAGGAAGgtaccgtatt132
10 (30896–0977)gtggttggtagGCTCATAAAG......AGGCCCACAGgtgagtcacc82
11 (31068–1162)tgttttggcagAAACTTCCAA...95
Primers for PCR
1, 25′-Forward -3′GTCACGTGACCGTCACTTCAC253
5′-Reverse-3′AGGCTGCTCACCCAAACGCGT
35′-Forward -3′AGGTGCAGCGAAGATGAAGGT267
5′-Reverse-3′GTCACTCATCCATCCTCCAC
45′-Forward -3′CAGACAGGTTCTGTGCTTGG175
5′-Reverse-3′GGACACAGATGGCATGAGG
4, 5, 6, 75′-Forward -3′CAGACAGGTTCTGTGCTTGG659
5′-Reverse-3′CACTGGGCTCAACTGCGTC
8, 95′-Forward -3′CTGAGCCTGGCTTCTCTTGG384
5′-Reverse-3′GAGTTCAAGGGAAAGCCCAG
10, 115′-Forward -3′CTGGGCTTTCCCTTGAACTC367
5′-Reverse-3′GTCCTACCAGCCAGCTTCTC