Primers and probes used for TaqMan allelic discrimination
| Target SNP | Primers and probes | |
|---|---|---|
| MGB, minor groove binder. | ||
| −1332 C/T | ||
| Primer | F | 5′-GGATGACACAGCCAGGAAGTCT-3′ |
| R | 5′-GCGTTTGGCTCCTTCTCATCT-3′ | |
| Probe | 5′-[VIC]CTACAGGTGATCATC[MGB]-3′ | |
| 5′-[FAM]CTACAGATGATCATCTC[MGB]-3′ | ||
| −499 A/T | ||
| Primer | F | 5′-CACTGCTCAAGGAAAAGAGGAT-3′ |
| R | 5′-AAGAAAGGCATTGGTAGCTTGATG-3′ | |
| Probe | 5′-[VIC]CATTTCATGTTCATGGAT[MGB]-3′ | |
| 5′-[FAM]AACATTTCATGCTCATGG[MGB]-3′ | ||
| −77 G/A | ||
| Primer | F | 5′-GCAATAGAGAAAGCAGAAATCTGATG-3′ |
| R | 5′-GGAGAGTAAAGATAAAATGTATATCTTACTTTGC-3′ | |
| Probe | 5′-[VIC]CATACTCCAAGTTCC[MGB]-3′ | |
| 5′-[FAM]AGCATACTTCAAGTTC[MGB]-3′ | ||
| +5080 T/C | ||
| Primer | F | 5′-ACATGGTGACCCAGGCATCT-3′ |
| R | 5′-TTCAGGAATCAAAATGGCAACAT-3′ | |
| Probe | 5′-[VIC]AGCTTCAAACAGATACA[MGB]-3′ | |
| 5′-[FAM]CTTCAAGCAGATACAG[MGB]-3′ | ||
| +13924 T/A | ||
| Primer | F | 5′-GCTGTTACACCAAAAGATGCACAT-3′ |
| R | 5′-CGGGATTGGAGAACAAACTCA-3′ | |
| Probe | 5′-[VIC]AGAGTCCTGTAACTTT[MGB]-3′ | |
| 5′-[FAM]AGTCCTGTTACTTTATT[MGB]-3′ | ||
| +20730 A/G | ||
| Primer | F | 5′-CAACACTATAAGTGGGTGCTTTAACG-3′ |
| R | 5′-TGCAGAGGGCCCCAAAG-3′ | |
| Probe | 5′-[VIC]AGGTCAAACAAAGTGG[MGB]-3′ | |
| 5′-[FAM] AGGTCAGACAAAGTG [MGB]-3′ | ||
| +20758 C/T | ||
| Primer | F | 5′-CAAAGTGGTGCCATCATCCA-3′ |
| R | 5′-TTGGACAGCTCCTCTAGTTCTTGAG-3′ | |
| Probe | 5′-[VIC]ACAGTCGCTTTGGG[MGB]-3′ | |
| 5′-[FAM]ACAGTGGCTTTGGG[MGB]-3′ | ||
| +25133 C/T | ||
| Primer | F | 5′-CACCGTGGGAAAGGACACTT-3′ |
| R | 5′-GGATCCCAGAGAAGGATTTGG-3′ | |
| Probe | 5′-[VIC]TGGACAACGCAGCC[MGB]-3′ | |
| 5′-[FAM]CTGGACAATGCAGC[MGB]-3′ | ||