Table 1

Primers used to amplify the EIF2AK3 gene

Exon	Forward primer	Reverse primer	Product size (bp)	Annealing temperature (°C)
Amplification of exon 1 required the addition of 5% dimethyl sulphoxide to the reaction mixture.
1	Gagaggcaggcgtcagtg	cgcgcgtaaacaagttgc	403	58.9
2	Tgagcatgtgggataagtgc	tgccctaaagggacacaaac	333	60
3	Tcaggatcaagactccagctc	tgacaacctcaggggaaaat	448	59.7
4	Ggagttggtaatctaactgatgc	ccaacagcaacattatctgaa	328	62.5
5	Gccctcttgtggcataaatc	ctgggagaggaagaaccgta	449	58
6	Tacttggggctctcagcttg	ggcactcctgaagtaggaagg	374	58
7	Ccctccctgtttttgttgaa	gggcaaagacagtcaggatt	389	60
8	Ctgggccatttgtttaactt	tgaaattgtctcccaagatg	384	55.6
9	Tagttaaagacgggcctatt	caagagtagctttggtggag	396	56
10	Aagactggagggatagcagt	agatcttaggtcatttcttctttg	371	58.1
11	Tgaactgattttcacattaccac	aattggcagcacttagaacc	340	58
12	Gccttcagggttgtcttact	cattgtaatcacacaagcaaa	384	56
13	Acagagggtgcagttcaggt	cacaatggttgccaatatgc	357	58
13	Aaggtcaagggagagaacct	acctctgctctcagatgctt	555	58
14	Catgcacacccactgtactt	ctggaacactactgccagttt	348	56
15	Ctttgggattcaataatgct	ccaatctgctggtattaagaa	288	57.5
16	Tgtggaatctgtgggatgtg	tgctaaggaccgcttacgtt	356	56
17	Ttttgccagcactgatttta	tttcaagtctgcaattttgg	367	58.2