Primer sets and pools used for DJ-1 gene dosage analysis
| Primer | Sequence | Product (bp) |
|---|---|---|
| Primers were pooled in sets of two DJ-1 exons and the control. The sets and primer concentrations were as follows. Set 1: exon 4 (0.6 µmol/l), exon 2 (0.8 µmol/l), and control (8 µmol/l); set 2: exon 1 (0.8 µmol/l), exon 6 (0.6 µmol/l), and control (0.8 µmol/l); set 3; exon 5 (0.8 µmol/l), exon 7 (0.8 µmol/l), and control (0.8 µmol/l); and set 4: exon 3 (1.2 μmol/l) and control (0.6 µmol/l). | ||
| DJ-1 E1 F | [Hex] 5′-GCTGTCCAGCTAGAAACTCC | 302 |
| DJ-1 E1 R | 5′-GACACCGTCCAGCACCC | |
| DJ-1 E2 F | [Hex] 5′-CTCTGCTTGAAAATGCTCC | 392 |
| DJ-1 E2 R | 5′-GGCAAGACATTAACAAGCG | |
| DJ-1 E3 F | [Hex] 5′-TTAAAGACAGTGTTACTCTGAATT | 388 |
| DJ-1 E3 R | 5′-CATCCAGCCACCCACTTAC | |
| DJ-1 E4 F | [Hex] 5′-GGCTATCTCCTGTACTTCCC | 297 |
| DJ-1 E4 R | 5′-TCACAGCCTCCTCCCGAA | |
| DJ-1 E5 F | [Hex] 5′-GTGAGTGATTGGTTAGTGGC | 293 |
| DJ-1 E5 R | 5′-ATCTCTGAAATGACACCAACTT | |
| DJ-1 E6 F | [Hex] 5′-CTCAAGCAATTTTTCTACCT | 367 |
| DJ-1 E6 R | 5′-GAGGCTGAGAGAGAAGAATCG | |
| DJ-1 E7 F | [Hex] 5′-CGT CTTTCTCGTCACATAGC | 360 |
| DJ-1 E7 R | 5′-TGTGACTTCCATACTTCCGC | |
| Control F | [Hex] 5′-ACGTTCCTGATAATGGGATC | 328 |
| Control R | 5′-CCTCTCTCTACCAAGTGAGG | |