Primers and PCR conditions for NSD1 analysis
| Exon | Sequence of primers | PCR |
|---|---|---|
| 2.1 | 5′GAGTCAGATGGCCTATTAACTC3′ and 5′GAATCTACATCTGCATCGTCC3′ | 56°C |
| 2.2 | 5′GTAACAAAGACTATCAAGAATGGC3′ and 5′GTTTCCCTTTAAGTGGCCTG3′ | 56°C |
| 3 | 5′CAGAAGGCTAATAGGAATGAC3′ and 5′GTTATTCTAAAGGCAACTGCC3′ | 56°C |
| 4 | 5′GCTGTTCTCTTAATGATGATGAGAAG3′ and 5′TTCTTACTTGCTTCTGTCACTC3′ | 56°C |
| 5.1 | 5′GACAGAGCAAGACTCTGTC3′ and 5′CTGTGAGGCTATTTGCTATCC3′ | 56°C |
| 5.2 | 5′TCCAGAGAACCTTGGCCTAAAC3′ and 5′TCCAGGCTCTGCACTCTTAG3′ | 60°C |
| 5.3 | 5′GAAGCCTCTCATTAGTAACTC3′ and 5′ATGGCTTTGATGTTCCAGAG3′ | 56°C |
| 5.4 | 5′ATCCGAGTTGAAGGAACTCTC3′ and 5′CACACTTGGAAGCTGATTCAG3′ | 56°C |
| 5.5 | 5′CTTCATCCAAATTGCGAGATGC3′ and 5′CAAGTATGCTTGCTGAAGGAG3′ | 56°C |
| 5.6 | 5′ACCTCGTAAGCGCATGAACAG3′ and 5′CTTCACTTTACCATTACAACAGACC3′ | 60°C |
| 6 | 5′GGGAGTATCAGATGGTCT3′ and 5′GGACCAGTGAAAGTTTGCTG3′ | 56°C |
| 7 | 5′ACAATTTTGGCCTGTGGACTC3′ and 5′CATATCTGCTCAAATACTGAGAC3′ | 56°C |
| 8 | 5′AATTAACTTGTGCCCAGTTTCTAA3′ and 5′CATCAATGTTATAACTGCTACTC3′ | 56°C |
| 9 | 5′TGGCAGCTGACAATTCAGAC3′ and 5′CTTCAAACTTACTACTGCATTACG3′ | 56°C |
| 10 | 5′GGACATGTGTGTTAGTAGCCAGC3′ and 5′GGATGTGGCCTCTGGCGTG3′ | 55°C |
| 11 | 5′GGGTCAAATGGAAGAGACATC3′ and 5′CCATCATAAAGAGATGGAGTGG3′ | 56°C |
| 12 | 5′TTACTTTAACCCACTGACACTGG3′ and 5′AACTAGCCCAGTGTTGCCAC3′ | 55°C |
| 13 | 5′CGATGTCAAACCGATCAGTCC3′ and 5′CACAGCGAGACTCTGTCTC3′ | 60°C |
| 14 | 5′CCATCATCTTAGTGGTCATTCC3′ and 5′CCTGAATGGCAGATGAATAGTATC3′ | 56°C |
| 15 | 5′CACATACATGACTTGCAGTC3′ and 5′CAGTGTATCTGAGAGGTCTC3′ | 56°C |
| 16 | 5′GCCTTGCAGCCTTCTAGAGG3′ and 5′CAGACACTCAGGTAACAGAGGTC3′ | 60°C |
| 17 | 5′GAAGTGACTTGTGCTGTCTG3′ and 5′TTCTCTTCTAGAGAAGGTCCC3′ | 56°C |
| 18 | 5′CGTGAATTGTCTTCTGCTGAC3′ and 5′GATCAATGATATCAAGCAACTGC3′ | 56°C |
| 19 | 5′TTTGCCATTAAGTCAGGAGG3′ and 5′CCTACAAATACTATGGCTGG3′ | 56°C |
| 20 | 5′ACAGCAGAGGTCTCAGGAAG3′ and 5′GAAATTCAGATGTCAGCTGCAG3′ | 60°C |
| 21 | 5′TCTCTTGGGAGTTGGTATCC3′ and 5′CACCACTAATCCCAAAGCAG3′ | 56°C |
| 22 | 5′GTGTTCACAGAATGCTGACTG3′ and 5′GAGTATGATGGAGAGAACGAG3′ | 56°C |
| 23.1 | 5′TAGCCTTGGCCCATGTGATATG3′ and 5′TAGCAGTGGCCTCTGACAAG3′ | 60°C |
| 23.2 | 5′AGAGCAATCAACAGGAATGGC3′ and 5′CTGATAGTACTTTCTCAGGAGG3′ | 56°C |
| 23.3 | 5′ACTTCAGACAGGCCTACTGAC3′ and 5′GGCTGAGAAAGAAGTCTGGC3′ | 60°C |
| 23.4 | 5′GATCCTCTTCAGACATCTGGG3′ and 5′GCAGCTTGTTTGTTCATGTGAC3′ | 60°C |