Primers and PCR conditions required for the amplification of the G6S gene
| Exon | Primers | Sequence | Product size (bp) | MgCl2 concentration (mmol/l) | Annealing temperature (°C) |
|---|---|---|---|---|---|
| 1 | SFD1(+) | 5‘-CGCCCTTCAGCTACCTGTG-3‘ | 333 | 1.5 | 66 |
| SFD1(−) | 5‘-ACCGGGAAAGAGAGCAACAA-3‘ | ||||
| 2 | SFD2(+) | 5‘-CAGGAATTCTGGGCTGTGTG-3‘ | 260 | 1.5 | 66 |
| SFD2(−) | 5‘-CCTTCCTTCAGATCAGGCATTT-3‘ | ||||
| 3 | SFD3(+) | 5‘-AAAGCAGCCCTGAAGCATGT-3‘ | 504 | 1.5 | 66 |
| SFD3(−) | 5‘-TGGGCAGATTCACAGAAAACA-3‘ | ||||
| 4 | SFD4(+) | 5‘-CAGGCCAGATTTGGCTCACT-3‘ | 332 | 1.0 | 66 |
| SFD4(−) | 5‘-TGCATCTGTAGGGCTGCATAA-3‘ | ||||
| 5 | SFD5(+) | 5‘-GTTTAGCCCAGCACGGATTC-3‘ | 327 | 1.0 | 66 |
| SFD5(−) | 5‘-GCTGGAAATACTCTTGGGCAGT-3‘ | ||||
| 6 | SFD6(+) | 5‘-CCCTGTACTGTCTGCTCACCA-3‘ | 341 | 1.0 | 66 |
| SFD6(−) | 5‘-ATGGCTCAAAGGCTTCCTGA-3‘ | ||||
| 7 | SFD7(+) | 5‘-GGAACACAGGAAGGGAGAGAA-3‘ | 311 | 1.0 | 62 |
| SFD7(−) | 5‘-TGAGCTGATTTGCAGGGTCT-3‘ | ||||
| 8 | SFD8(+) | 5‘-CGGGGGTGTCTTTGTTTTTG-3‘ | 294 | 1.5 | 62 |
| SFD8(−) | 5‘-GGGCTCATTGGGTGAAAAAG-3‘ | ||||
| 9 | SFD9(+) | 5‘-CCCAGGCAAATTTCAGCTTT-3‘ | 359 | 1.5 | 64 |
| SFD9(−) | 5‘-CAGCCCTAGTGGGAAGAGGA-3‘ | ||||
| 10 | SFD10(+) | 5‘-CCAAAGTATGGGCTGTATAAAAGG-3‘ | 355 | 1.0 | 62 |
| SFD10(−) | 5‘-CAAGATTTTCTGCCCCCAAA-3‘ | ||||
| 11 | SFD11(+) | 5‘-TGACTCCCAGGTCCCCTTTA-3‘ | 263 | 1.5 | 66 |
| SFD11(−) | 5‘-AACCAGCACCTTGCCATGTT-3‘ | ||||
| 12 | SFD12(+) | 5‘-CGATTGCCCTTCTAGGCAAC-3‘ | 282 | 1.5 | 66 |
| SFD12(−) | 5‘-GCAGCCTTGGAATTGCTCTT-3‘ | ||||
| 13 | SFD13(+) | 5‘-CCTCCTCCCTTCCCCTTTAG-3‘ | 331 | 1.5 | 64 |
| SFD13(−) | 5‘-TCCCTTTGCCTTCAAGAACA-3‘ | ||||
| 14 | SFD14(+) | 5‘-CACCCCTTCCTGCTATCCTTT-3‘ | 292 | 1.5 | 66 |
| SFD14(−) | 5‘-GGACTTAAAGCCAGCCCAGA-3‘ |