WSCR gene primer sequences
| Gene | Primer sequence (5‘-3‘) | Annealing temperature | Size of product (bp) |
|---|---|---|---|
| FKBP6 X6 | F CTCTTGACCATCTGCCTTCCC | 60°C | 310 |
| R CCTGGGTGTTTATTCCCAAGTCAC | |||
| BCL7B | F CGCGGTCAGAAAGCTAGCACC | 60°C | 302 |
| R ACTGCTGCCTGGAGGTCACAG | |||
| STX1A | F TCGCCTAGAAGCCACCCAAAC | 60°C | 280 |
| R CTGCCTGGGTCTGCTCCTCGC | |||
| ELN X2 | F TCCATGTAATTGTGGGTTTTGCC | 60°C | 256 |
| R CAATGTTCCTACCTTCTGTAGTG | |||
| ELN X34 | F GATTAGAGCCGAAACTGAGAG | 60°C | 261 |
| R AGGTTACAGCAGGGCGAGAGC | |||
| LIMK1GT 10 | F AGTCTTCTTTGCGGGCTATGTTA | 60°C | 180 |
| R TGGGGCAGGAGAATGATGTG | |||
| LIMK1 3′ | F CCGACTGAGCCAGGGCCACTC | 65°C | 241 |
| R AGGTTACAGCAGGGCGAGAGC | |||
| WBSCR1 X2 | F CCCGCGGCAGTGCTGGTGGCCAT | 60°C | 350 |
| R GCCACTTAGACCAGGCAAATGTT | |||
| WBSCR5 5′ | F TCATAGCTGAGCCTGTCCGGC | 60°C | 200 |
| R AGGCTTACCTGAGAGTCCAGGGT | |||
| RFC2 3′ | F GTGGCCAGTTAGAGCAGAGAC | 60°C | 500 |
| R GTCCCAGAAGCAAACATGCAAGT | |||
| CYLN2 | F AAGAGAAATCCCTGTCGGATC | 60°C | 172 |
| R TTGTCAGGGCAGCTCCTCATG | |||
| GTF2IRD1 | F AATGGCCAATGTACATGGTGG | 54°C | 204 |
| R GCCTTGGTGGCAGGAATATAG | |||
| GTF2I X1 | F CTTCTTACTTCTCCTGCACAG | 60°C | 131 |
| R AAATCAACCTTATCTACAGTG | |||
| GTF2I X10 | F AGCTTCCAAATAGAAGCGTTGTA | 60°C | 160 |
| R TTCCCATAGGCTTTATTAATCAG | |||
| GTF2I 3′UTR | F C TACATAACCT AGATAACCTA AC | 60°C | 170 |
| R TATGCAGGGGGTTCAGATGGACA | |||
| NCF1 X2 | F CCTTTCTGCAATCCAGGACAACC | 60°C | 200 |
| R ATTTCACCAGGAACATGTACACC | |||
| NCF1 X6 | F GGGGACGTGGTGGAGGTCGTA | 60°C | 210 |
| R CCACAGGGTGCACACTAAGAC | |||
| CFX11 | F CAACTGTGGTTAAAGCAATAGTGT | 60°C | 400 |
| R GCACAGATTCTGAGTAACCATAAT | |||
| D7S669 | F ATGCAACCTACCCTCAAATG | 60°C | 120 |
| R TACGGTTTACCCACATTGCTAT |