Table 3

Primer sequences

Exon	Primer sequences	Annealing temp (°C)	Reference
2/3	Forward: 5′-ggatccccacttttcctctt-3′	57
	Reverse: 5′-agcatcaaatcatccattgc-3′
4	Forward: 5′-gacctggtcctctgactgct-3′	54
	Reverse: 5′-atacggccaggcattgaagt-3′
5/6	Forward: 5′-tgccctgactttcaactctgt-3′	54
	Reverse: 5′-ttaacccctcctcccagaga-3′
7	Forward: 5′-tgccacaggtctccccaagg-3′	55	Evans et al⁵³
	Reverse: 5′-aggggtcagcggcaagcaga-3′
8/9	Forward: 5′-caagggtggttgggagtaga-3′	54
	Reverse: 5′-acttgataagaggtcccaag-3′
10	Forward: 5′-atgttgcttttgatccgtca-3′	54
	Reverse: 5′-ctttccaacctaggaaggca-3′
11	Forward: 5′-agccacctgaactcaaaaa-3′	55	Evans et al⁵³
	Reverse: 5′-aatggcaggggagggagaga-3′