Table 1

Patient and RLGS analysis data for 13 HNSCC patients

					% of analyed loci methylated
Patient	Age (y)	Sex	Race	Tissue	Primary	Metastatic	*Primary only	†Metastatic only	‡Both	§NA	¶% lost in primary but not metastasis
Number of loci methylated in the primary tumour, but not the metastatic tumour.   †Number of loci methylated in the metastatic tumour, but not the primary tumour.   ‡Number of loci methylated in both the primary and metastatic tumours.   §Number of loci that were methylated in one tumour, but unable to be analysed in the other tumour RLGS profile.   ¶Number of loci methylated in the primary only divided by the number of loci methylated in primary only plus the number methylated in both primary and metastasis.   *An unpaired Z test test using the normal approximation to the binomial distribution to compare the frequencies of methylation between primary tumours and metastatic tumours indicates significantly more methylation in the metastatic tumours as a group (p<<0.001).
2	44	M	Bl	Tongue	2.1	1.9	6	12	16	0	27
4	56	M	Wh	Pharynx	1.2	0.57	13	6	1	0	93
7	52	M	Wh	Pharynx	1.3	1.2	14	14	8	0	64
8	46	M	Wh	Tonsil	0.76	0.67	11	8	3	0	79
11	67	M	Wh	Tonsil	0.09	0.36	1	5	1	0	50
12	65	F	Wh	Tonsil	1.4	1.6	5	16	8	1	38
14	65	M	Wh	Larynx	0.74	1.4	6	16	2	2	75
15	77	F	Wh	Tonsil	0.90	3.3	9	27	1	0	90
16	59	M	Wh	Tongue	0.74	1.9	2	12	4	0	33
23	42	M	Wh	Pharynx	2.0	3.1	8	14	7	3	53
28	48	M	Wh	Tongue	0.46	1.2	4	15	2	0	67
29	62	M	Wh	Tongue	1.2	1.9	2	11	10	0	17
32	65	M	Wh	Pharynx	1.3	1.8	12	17	7	1	63
Ave					**0.99	**1.47					58