Oligonucleotides used as primers for amplification of aldolase B exons
| Exon | Primer | Sequence (5`-3`) | Fragment size (bp) |
|---|---|---|---|
| For each of exons 5 and 9, two PCR fragments of different sizes were amplified, the longer ones being used for sequencing purposes. | |||
| 2 | E2+ | GTCATGCATCCATCTGAG | 319 |
| E2− | GTCTACTGAGTCTTCTGC | ||
| 3 | E3+ | GAGATGATGTGGAGAAGG | 373 |
| E3− | ATGTTCAGAGTGTTGGCC | ||
| 4 | E4+ | GTCCCTCGCACTAATACA | 258 |
| E4− | CCTTGCTTCCTTCTTTAC | ||
| 5 | E5+ | CTTGATGGCCTCTCAGAG | 214 |
| E5− | GCTCTGAAGAAAACTCTAG | ||
| 5 | E5+B | CTCAAGCACAGTGGATTG | 339 |
| E5− | GCTCTGAAGAAAACTCTAG | ||
| 6 | E6+ | GCAACTGAAGAATCTCCT | 230 |
| E6− | AGTAACAGCTGTTACCTA | ||
| 7 | E7+ | GTCAAGTGGCTCTATGAC | 294 |
| E7− | CTTGTGGCTCTCCAAAGA | ||
| 8 | E8+ | GCAGGGTATATAAGGTGG | 279 |
| E8− | GTAGATAAGAGGTGGCAG | ||
| 9 | A9+ | TGGTATCCCCAGCAATATTCAG | 299 |
| A9− | ACTAGAAGCACTGGAGCTAGGCT | ||
| 9 | E9+ | CATGAGAGGCAGACAGGGT | 390 |
| E9− | GCAAGGAAACTGCTGTGTG | ||