Table 2

Polymerase chain reaction primers and restriction digest fragment sizes used to screen the congenital and age related cataract populations for the four known Cx50 mutations

Mutation	Restriction site	Primers (sense/antisense)	Wild type (bp)	Mutant (bp)
R23T	Loss of HINf1	F5′-cccgcgttagcaaaaacagat-3′ and R5′-cacagaggccacagacaacat-3′	574, 132, 27	706, 27
P88S	Creates Mnl1	F5′-tctgggtgctgcagatcatc-3′ R5′-ccatgcggacgtagtgcac-3′	81	50, 31
E48K	Loss of FokI	F5′-cccgcgttagcaaaaacagat-3′ R5′-ccatgcggacgtagtgcac-3′	193, 172, 33	193, 205
I247M	Loss of HINf1	F5′-cactacttcctgtacgggttc-3′ R5′-ctcttgccctacgtaagcttgg-3′	610, 237	847