Lab-on-a-chip analysis
| Sample number | Mean | ||||||
|---|---|---|---|---|---|---|---|
| 1 | 2 | 3 | 4 | 5 | 6 | ||
| Following electrophoresis, the peak data areas from six samples (1–6) were imported into Excel from the Bioanalyzer software (Section A). | |||||||
| By dividing the area under each exon (specific peak is divided by the sum of the area of the control peaks), a ratio for each exon was obtained (Section B). | |||||||
| These ratios are were then normalised to 1.0 based on the mean ratio of samples known to be unaffected at that specific locus (Section C). | |||||||
| The normalised ratios of the exons that are duplicated are shown in bold, those of the deleted exons are in italics. | |||||||
| A | |||||||
| Exon 52 | 5.31 | 6.86 | 8.08 | 14.81 | 7.27 | 6.40 | — |
| Exon 62 | 12.66 | 8.14 | 13.68 | 12.16 | 5.71 | 5.08 | — |
| C1 | 16.77 | 12.11 | 14.50 | 18.43 | 8.84 | 7.06 | — |
| C2 | 16.12 | 8.86 | 11.54 | 13.42 | 7.03 | 5.69 | — |
| C3 | 17.74 | 12.14 | 13.73 | 18.08 | 8.61 | 6.82 | — |
| Exon 54 | 6.68 | 8.71 | 10.93 | 18.75 | 9.51 | 6.76 | — |
| Exon 49 | 7.32 | 4.34 | 10.18 | 12.46 | 6.60 | 4.88 | — |
| B | |||||||
| Exon 52 | 0.10 | 0.21 | 0.20 | 0.30 | 0.30 | 0.33 | 0.21 |
| Exon 62 | 0.25 | 0.25 | 0.34 | 0.24 | 0.23 | 0.26 | 0.25 |
| Exon 54 | 0.13 | 0.26 | 0.27 | 0.38 | 0.39 | 0.35 | 0.27 |
| Exon 49 | 0.14 | 0.13 | 0.26 | 0.25 | 0.27 | 0.25 | 0.25 |
| C | |||||||
| Exon 52 | 0.48 | 1.00 | 0.95 | 1.43 | 1.43 | 1.57 | — |
| Exon 62 | 1.00 | 1.00 | 1.40 | 0.96 | 0.92 | 1.04 | — |
| Exon 54 | 0.48 | 0.96 | 1.00 | 1.41 | 1.44 | 1.30 | — |
| Exon 49 | 0.56 | 0.52 | 1.04 | 1.00 | 1.08 | 1.00 | — |