Primer name | Sequence (5′-3′) | Position | Fragment size | Annealing temp | PCR buffer | |
---|---|---|---|---|---|---|
Buffer A: Red Taq buffer (Sigma, Zwijndrecht, The Netherlands); buffer A* is Red Taq buffer with final concentration of 1.2 mol/l Betain; buffer B: supertaq buffer (Sphaero Q, Leiden, The Netherlands). | ||||||
Gsh12 | F | GAGCACCTTGCCCGAGCCTTACC | 1496–1518 | 364 bp | 56 | A |
R | CTCCTTGTCATCGTTGGCTGAGG | 1838–1860 | ||||
Gsh13 | F | TCCGAGGATGAGGACTCCCTGTC | 1810–1832 | 370 bp | 56 | A |
R | CCCCATACAGTGTTAAACTTAA | 2159–2180 | ||||
Gsh14 | F | CAACTCATTCTTGTCGTATAAC | 2117–2138 | 374 bp | 55 | B |
R | GCAGGAGAAGATAGGGAGAGCAA | 2159–2180 | ||||
Gsh15 | F | AGGGCAGAGCTTAGAACACTAGA | 234–256 | 286 bp | 56 | A* |
R | AGTGCAGGTGCGAAGTGAC | 502–520 | ||||
Gsh16 | F | CCATTGGTGATGTCCGTGT | 427–445 | 302 bp | ND | ND |
R | CTGATGATGGTGATGGTGGT | 710–729 | ||||
Gsh17 | F | GTGAACCATGCGCATCATC | 673–691 | 249 bp | 56 | A* |
R | AAAGGCGCGGAGCGCAGGTG | 903–922 |