Table 1

Sequence of primers and probes for the SNPs used in this study

GeneSNP locationAmino acid alterationPrimers and probes†dbSNP ID
†WF, wild type forward primer; MF, mutation forward primer; WR, wild type reverse primer; MR, mutation reverse primer; P, probe. FAM, HEX, and TET indicate the different fluorescent labels.
‡HTR1A and HTR7 were genotyped using two different methods.
§Primers and probe designed for HTR1A were taken from published sequences.1
HTR1A‡Exon1/G402AArg134ArgWF1FAM5′- CGCCATCGCGCTGGACACG-3′–§
WR15′-CCCCGACTCTCCATTCACAC-3′
MFTET5′-CGCCATCGCGCTGGACACA-3′
MR5′-TGAGCAGCAGCGGGATGTAG-3′
WF25′-CGCTGTATCAGGTGCTCAAC-3′
WR25′-CCATGATCCTTGCTAATGGTG-3′
P5′-CTTGCACCTGTGCGCCATCGCGCTGGACAG-3′
HTR2A Exon3/C62428T–WFFAM5′-TCTACAGTAATGACTTTAACTGC-3′rs6313
MFTET5′-TCTACAGTAATGACTTTAACTGT-3′
WR5′-CAGCTATGGCAAGTGAC-3′
HTR2C Exon4/C12GLeu4ValWF5′-TCTCTCCCCAGAAAGGATGA-3′rs2228669
WR5′-TTGAGCAAGCTACCAAGCAA-3′
P5′-CTGAAGCAATCATGGTGAAC-3′
HTR5A Exon1/A12T–WFFAM5′-TGACCCAGAGATGGATTTACAA-3′rs6320
WR5′-GCCCAGCAAGGTGAGAATAA-3′
MFHEX5′-TGACCCAGAGATGGATTTACAT-3′
HTR6 Exon1/C734T–WFFAM5′-CATGCTGAACGCGCTGTGC-3′rs1805054
MFHEX5′-CATGCTGAACGCGCTGTGT -3′
WR5′-CGTCTCCGAGGCCTGACT-3′
HTR7‡Exon1/G3217A–WF1FAM5′-GGATATTTTTAGGCTAATGG-3′rs1935347
MFTET5′-GGATATTTTTAGGCTAATGA-3′
WR15′-GGGTGTTGTTTTCCTG-3′
WF25′-AGGGGCTGCCAGTTGTATTT-3′
WR25′-CAAACATTCAGGCCATAGCA-3′
P5′-TAATTACAGAATATCTACTTCATTAAGGA-3′