Table 1

PCR primers and conditions

PolymorphismPrimer sequenceTmTouch-downAnnealingMg2+Amplicon size
Pro12AlaForward inner primer (C allele): 5′-GAAACTCTGGGAGATTCTCCTATTGTCC65°CYes61°C0.5 mmol/l221 bp (C allele)
Reverse inner primer (G allele): 5′-GTATCAGTGAAGGAATCGCTTTCAGC65°C288 bp (G allele)
Forward outer primer: 5′-AACTTTTTGTCACAGCTGGCTCCTAATA65°C455 bp (from two outer primers)
Reverse outer primer: 5′-CAACGAGCTAAGCATTAAAATACTGGA65°C
C161TForward inner primer (C allele): 5′-AGACCTCAGACAGATTGTCACGGAAAAC69°CNo65°C0.5 mmol/l165 bp (C allele)
Reverse inner primer (T allele): 5′-TCTTGATCACCTGCAGTAGCTGCCCA71°C202 bp (T allele)
Forward outer primer: 5′-ATTATTCTCAGTGGAGACCGCCCAGGTT70°C312 bp (from two outer primers)
Reverse outer primer: 5′-AGTGCAACTGGAAGAAGGGAAATGTTGG70°C