RT-PCR primers
| Primer set | Region amplified | Forward primer | Reverse primer | Product size (bp) |
|---|---|---|---|---|
| 1 | 635–3206 | 5′ AGAGCCGGGAGGAGCGTGTTC 3′ | 5′ TGACAATGATGGGCGGATCGTC 3′ | 2593 |
| 2 | 2987–5321 | 5′ TACACCAAGAAGCAGGCGTTCC 3′ | 5′ GATGATGACCCGCAGCTCGTA 3′ | 2345 |
| 3 | 4986–6225 | 5′ GCAAAGACGGCAAAGTGGACGG 3′ | 5′ AACAGCGCCAGCACGATCTTGA 3′ | 1261 |
| 4 | 193–710 | 5′ AGTGACTTTCCGAGGGCAATCC 3′ | 5′ CAGCACCGCCGGTTCATC 3′ | 535 |
| 5 | 283–2049 | 5′ GGCCAGCAGCATCGACAG 3′ | 5′ GACAGGCCATCAACTTCGTTCC 3′ | 1788 |
| 6 | 1840–3206 | 5′ GGGCCTGGCTGTGGAGATCGT 3′ | 5′ TGACAATGATGGGCGGATCGTC 3′ | 1388 |
| 7 | 2654–4206 | 5′ CAGCACAGCATTCCCGACATC 3′ | 5′ CCCTTCAGGCCCTCGGTATTGT 3′ | 1574 |
| 8 | 3976–5082 | 5′ GGTGAAGCTGGACGCGACTT 3′ | 5′ GTGGGCTTCCTCTGACCGTTCT 3′ | 1128 |
| 9 | 4706–6225 | 5′ CGCGACAAGGAGAACTACATCT 3′ | 5′ CGCCAGCACGATCTTGA 3′ | 1536 |
| 10 | 280–711 | 5′ GGTGGCCAGCAGCATCGACA 3′ | 5′ ATCTCCAGCACCGCCGGTTCAT 3′ | 431 |
| 11 | 464–711 | 5′ TGCGTGGAGGTCCGGTATCAGG 3′ | 5′ CTCCAGCACCGCCGGTTCAT 3′ | 247 |
| 12 | 634–904 | 5′ GAGAGCCGGGAGGAGCGTGTTC 3′ | 5′ CTGCCGGGCCTCGATCACC 3′ | 270 |
| 13 | 1300–1654 | 5′ GGCCAATGAGACCGACGAAGA 3′ | 5′ GTCAATGAAGTGGGTGCCGATG 3′ | 354 |
| 14 | 5323–5823 | 5′ CGAGCTGCGGGTCATCATCT 3′ | 5′ TTGCCCGTGAGCTCAAACTCAT 3′ | 522 |
| 15 | 2353–5886 | 5′ CGAGCTGCGGGTCATCATCT 3′ | 5′ CCAGGCCCACTGGGTTCTTCT 3′ | 584 |
| 16 | 4903–5404 | 5′ CGGCATCGCCCAGACCTAC 3′ | 5′ GCCACCCCCTCACGAAGATG 3′ | 521 |
| 17 | 4431–4935 | 5′ AGACCGGGGATGATGAGGATG 3′ | 5′ GGGGTCCCGCCAGATATTGT 3′ | 524 |
| 18 | 4214–4614 | 5′ GAGGGCCTGAAGGGGTCAAT 3′ | 5′ GTGGCCCGGACCACATAGA 3′ | 419 |
| 19 | 3893–4412 | 5′ AATGGGGGCTCCTCCTCTCA 3′ | 5′ TTGCCCCGAAGCAAGTTGAA 3′ | 539 |
| 20 | 3545–3975 | 5′ TGGGGCCTACGGGACCTAAA 3′ | 5′ AGTCGCGTCCAGCTTCACCA 3′ | 450 |
| 21 | 3074–3609 | 5′ CCCTTTGCCCGCGTCTTCT 3′ | 5′ CACCCCCTTCCCTGCACACT 3′ | 555 |
| 22 | 2826–3264 | 5′ GCTCGGCAGGCTGGACAGT 3′ | 5′ GAAGGTCCGGCCCATGAAGT 3′ | 458 |
| 23 | 2482–2897 | 5′ ATCCCGCACCAGGCTTGAC 3′ | 5′ GCAGGCCGCACAGGAACTC 3′ | 434 |
| 24 | 2101–2536 | 5′ GCCGGGGGATGAGGAAGA 3′ | 5′ GCCTGCTGCCCCATGTTT 3′ | 453 |
| 25 | 1550–2140 | 5′ GAGCCCCTGTGGAATGAGCA 3′ | 5′ CACCGGCCTCGTCATCACTT 3′ | 610 |
| 26 | 1208–1665 | 5′ GACCCCGATGACATCTCCTC 3′ | 5′ CTTGCGCAGGTCAATGAAGT 3′ | 477 |
| 27 | 637–1215 | 5′ AGCCGGGAGGAGCGTGTT 3′ | 5′ CAGCCCCGAGGAGATGTCAT 3′ | 598 |
| 28 | 30–664 | 5′ AGGCAGCGGCAGAGAAGAGAG 3′ | 5′ CCGGTTTTTGCCGAGCTTC 3′ | 653 |
| 29 | 4684–5927 | 5′ CCGGCTAGGCAAGACTGACAT 3′ | 5′ GGCCGGTTGGGTTTCTCTAG 3′ | 1263 |