PCR/restriction digest analysis of sequence family variants in the AZFc region
| STS | Restriction enzyme | BAC clones | Fragment sizes (bp) after restriction |
|---|---|---|---|
| *The submitted sequence of the chromosome 1 derived BAC clone RP11-560I18 (AC053522) does not show a Tsp509I restriction site within the genomic fragment amplified by the primer pair SKY10. Restriction analysis of fragments amplified from male and female genomic DNA, from a somatic cell hybrid line containing chromosome 1 as the only chromosome of human origin, and from the BAC RP11-560I18 also shows two fragments of ∼180 bp and ∼155 bp, indicating a sequence error in the complete sequence of the BAC clone. | |||
| SKY10 | Tsp509I | 487K20 | 279, 50 |
| 70G12 | 329 | ||
| 560I18 | 329* | ||
| SKY11 | NlaIII | 245K04 | 217, 154, 79, 19 |
| 506M09 | 233, 221, 15 | ||
| SKY12 | MseI | 245K04 | 88, 57, 39, 32 |
| 506M09 | 145, 39, 32 | ||
| SKY13 | Cac8I/TfiI | 100J21 | 97, 83, 23 |
| 589P14 | 175, 23 | ||
| 251M08 | 97, 50, 33, 23 | ||