Table 2

PCR primers for SSCP

Fragment	Primer	Sequence	Annealing T
Fragment 1
1	AB1F	GCCATGCGCGCGCTGCCCTAACG	60°C
	SAB1B1	GAGGTTCGCCAGGAGCCCAACG
2	SAB1F2	ACA GCG CTA GAC GTC TCC C	60°C
	SAB1B2	AAG GGG ATG CCA AGC AGA GG
3	SAB1F3	CCA CGT GTG CTG GGC CTG G	60°C
	AB5B	GGCACTGGAGGGCTGGGCCGC
Fragment 2
1	AB5F	CAGGGCTGGTGCCTGTGTGGGGCG	60°C
	SAB5B1	CACATAGCGATGCGAGGCAGCCGG
2	SAB5F2	GCT CCG CCG AGG TGG ATG CC	62°C
	SAB5B2	TGT CCG AGG GGC AGA GCG GG
3	SAB5F3	TGG TTC AGG CCT GGA GGC	56°C
	SAB5B3	TGG CAG CTC TCC AGG CTG
4	SAB5F4	TCA CCA GGA GCC TAG ACG	52°C
	SAB5B4	AAT ACC ATG ACC TCC ACG
5	SAB5F5	GCA GGA CGG CCT CTC AGC CCC	60°C
	AB10B	GGCCTGGGGGTGGCAAGAGGCGTC
Fragment 3
1	AB10F	GGA CGC CTC TTG CCA CCC CC	60°C
	SAB10B1	GCT GGC AAG TGG GGC AGC CA
2	SAB10F2	TCT CCG CCA ACG CCT CGT CA	60°C
	SAB10B2	GTG GCG TTG GCA CCA GAG TC
3	SAB10F3	CAG CCT TGG TGC TCC AGG TG	64°C
	SAB10B3	GAC TCC CTG CAG TAC ACG GG
4	SAB10F4	GAG CCC ATC TGT GGC CTC C	60°C
	SAB10B4	GTG GAG ACC TGC AGA CCC TG
5	SAB10F5	CGT GAA CTA CAA CGT AAC CG	62°C
	SAB10B5	GCA GCG TAG GTG TGC ATG AC
6	SAB10F6	GGT GCT GGT GGA GCA CAA TG	60°C
	SAB10B6	CGA GGC ATA GGT GTG GTT GG
7	SAB10F7	CTG TCC TGA CCC AGA GCC	58°C
	SAB10B7	GCA GGT ACA CAT GCT CCA C
8	SAB10F8	GTG GAC CTT CGA CAT GGG	58°C
	SAB10B8	CAC GGT CGT GTT GGA GGA G
9	SAB10F9	CCTCTTCGACTGGACCTTCGGGG	60°C
	AB15B1	CTGGCACGGGTGGGGGCGGCTTCC
Fragment 5
1	AB15F2	GGGATGGCACCAACGTCTC	62°C
	SAB15B1	CCT CCT CCA AGG ACC AAG TG
2	SAB15F2	CTG GTGGCA GTG GTG TCG	60°C
	SAB15B2	CAG CAC CAG CTC ACA TTG G
3	SAB15F3	TGC CCT TTT GGG GGC AGC	63°C
	SAB15B3	GGC GGA AGG TGA CAG CTG AGC
4	SAB15F4	CAG ATC CTG CTG GCT GCC G	65°C
	SAB15B4	GTA GGC GAC CCG AGA GCC GC
5	SAB15F5	TTC ACA GCC CGC GTG CAG C	63°C
	SAB15B5	GGT GGC GGC CTC AAA CTG C
6	SAB15F6	CTG CTT CAC CAA CCG CTC GG	65°C
	30Ext	CCTCCAAGTAGTTGCGCTGTGATCG
Fragment 6
1	FQF26	AGC GCA ACT ACT TGG AGG CCC	62°C
	SAB26B1	ATG AGC CAC CCT CAA TGA TGG
2	SAB26F2	CAG GCC AAT GTG ACG GTG G	60°C
	SAB26B2	CCT TCC ACA CGG TCA GGC TG
3	SAB26F3	CGG CTG GCG TGG AGT ACA CC	60°C
	SAB26B3	CAG TCG CAT GCC TGC ACT GC
4	SAB26F4	TGG ATG AGA CCA CCA CAT CC	62°C
	SAB26B4	CAG CAG CAG GGC GTA CAC C
5	SAB26F5	CTT CGA ATG CAC GGG CTG G	62°C
	SAB26B5	GTG CAG CCA GAC TGT GAG CC
6	SAB26F6	TCA ACA GGT CTT TGG CCA TC	62°C
	SAB26B6	CGA TCT GCT GGA TGT CAT CC
7	SAB26F7	GAC TCT GGT GTC CCT GAG GG	60°C
	SAB26B7	CAT CCG AGA TGG TGA CTC G
8	SAB26F8	GCC AGC TCG GAC GTG CGG	60°C
	SAB26B8	GTC ACT GAG GTT GGC CAG G
9	SAB26F9	CCA CTT CTC CAT CCC CGA GG	62°C
	AB21B	ACC ACA ACG GAG TTG GCG G
Fragment 7
1	AB21F	CAGCTCCGCCAACTCCGCCAACT	62°C
	SAB21B1	GCT GGG TCT CTG CTC CCC GG
2	SAB21F2	CTG ACC ACC GGC CCT ACA CC	62°C
	SAB21B2	AGC GGA CAT GGC TTG GGG C
3	SAB21F3	CCT GGA GGA GAC CTC GCC	62°C
	SAB21B3	GTG GCC GCT CCG GCT GTC CAC C
4	SAB21F4	ATC CTC GTC AAG ACA GGC	54°C
	SAB21B4	GAA AGC CAG TCA TTG ACC
5	SAB21F5	GAC CTG CAG ACG GCA CGC	64°C
	SAB21B5	AGT CGC CAA CAG CCC CG
6	SAB21F6	CTG CCT CTT CCT GGG CGC C	66°C
	SAB21B6	GCG TGG AGG CCT GAG AAC G
7	SAB21F7	TGG ACT CGT CCG TGC TGG	62°C
	SAB21B7	GGA TCA GGT CTT CAT CTG ATG C
8	SAB21F8	CCC CTA CTC GCC TGC CAA ATC C	68°C
	SAB21B8	TCC TGG ACA GCC TCG CTG CC
9	SAB21F9	GAC GCT GGC GCT GCA GAG GC	68°C
	AB36B	GGACAGGAGCCACGCAACACTCAC
Fragment 9
1	AB42F	CCTCTCGCTGCCTCTGCTCACCTC	62°C
	SAB42B1	AGC GAG GCC GCC AGG CCA CG
2	SAB42F2	CCA GGT GGC GCA GCT GAG CTC	62°C
	SAB42B2	CAG GAC TCG GCA GGA CAC AGG
3	SAB42F3	TGG TGC TGT GCC CTG GGA CTG G	62°C
	SAB42B3	GAG GGC AGC GGC TCC ATC CCT TC
4	SAB42F4	GGA GT CCG CCA CAA AGT CC	64°C
	SAB42B4	CCT TGC AGG CTG TGC AGC
5	SAB42F5	CAA CCA GGC CAC AGA GGA CG	62°C
	SAB42B5	CTC GGC CTT GAC AGC GGC
6	SAB42F6	AGT CGG AGT GGA CAC CGC	62°C
	SAB42B6	CAT CTG CCC AGG GGG TGG
7	SAB42F7	CTC AGG TAC AGC GGG CTG TGC	62°C
	AB46B	ATAACGCCACCACACCTACCAAGC