Table 1

cDNA probes used to study the CBP gene in RTS patients

Probe	Position	Exons hybridised	Fragment size (bp)	Sequence 5`→3`	PCR conditions MgCl₂ - Annealing T
Positions of the primers are given relative to the start codon. The first primer of each set has a forward orientation, the second reverse.
P1	−796, −777	1, 2	933	GAGGAATCAACAGCCGCCAT	2.5 mmol/l - 60°C
	+117, +137			GGTATCAGCTCATCAGGAAGA

P2	+33, +52	2-4	995	CCCCAAAAGAGCCAAACTCA	2 mmol/l - 55°C
	+1008, +1027			CTGTTGCAATTGCTTGTGTG

P3	+949, +968	4-9	985	ACTTCAGTCACCAACGTGCC	1.5 mmol/l - 60°C
	+1914, +1933			CAGACTCGTACATGTCCCCT

P4	+1847, +1866	9-15	1139	CACCTGATCCCGCAGCTCTA	2 mmol/l - 60°C
	+2967, +2985			CACAGGTACGTCAGGTCCT

P5	+2741, +2759	14-22	1197	AGAGCACCCCTACAGTCCA	2 mmol/l - 60°C
	+3916, +3937			AGCAGTTGTCGCACACAAAACC

P6	+3756, +3775	21-29	1212	GGGTGACGACCCTTCACAGC	2 mmol/l - 60°C
	+4948, +4967			AGGGGGTCGGGGTCGACGAT

P7	+4836, +4855	29-31	1058	CAACGTGTCCAATGACCTGT	1.5 mmol/l - 55°C
	+5873, +5893			CTCGATCTGCCGAGCCGCTT

P8	+5742, +5761	31	1283	CGTGAGCATGTCACCAGCTG	2.5 mmol/l - 60°C
	+7005, +7024			TACTAAGGGACGTGGCGATC

P9	+6903, +6922	31	1333	GATGAAGCAGCAGATTGGGT	1.5 mmol/l - 60°C
	+8214, +8235			CCTTTTTGTCTGTTGCACACAG