Screening for eight candidate MSI target genes was performed in MSI positive CRCs. Primer sequences, length of PCR fragments, annealing temperatures, and MgCl2 concentrations are listed. Genes marked with • are from the search with keywords related to cancer, and genes marked with * from the tumour suppressor homology search
| Gene (repeat) (GenBank accession No) | Function | Primer sequence | Length of PCR fragment (bp) | Annealing °C | MgCl2 (mmol/ l) | Mutations |
|---|---|---|---|---|---|---|
| BCL10 (T7) | Apoptotic signalling gene | F: agc cac gaa caa cct ctc c | 240 | 59 | 1.25 | 0/29=0% |
| (AF097732)• | R: tgt aga tct ggt ggc aaa gg | |||||
| PTP-BAS (A8) | Protein tyrosine phosphatase | F: tgg gat ttc tgt cca tca aag | 250 | 61 | 2.5 | 0/30=0% |
| (D21209)• | R: caa cga cga tat gtg gca gt | |||||
| CtIP (A9) | CtBP (phosphoprotein) binding protein | F: ttg tcc cct tct ctt tta cag c | 274 | 61 | 2.5 | 25/109=22.9% |
| (U72066)• | R: ggg gct cca aat gtt tat ca | |||||
| BAI1 (C7) | Brain specific angiogenesis inhibitor | F: tct gcg tgt cct cct cct ac | 346 | 59 | 2.5 | 0/30=0% |
| (NM_001702)* | R: agg gca atg ttg cag aac tt | |||||
| P73 (C6) | p53 related protein | F: gct gat gag gac cac tac cg | 484 | 59 | 2.5 | 0/30=0% |
| (AF077616)* | R: ctt cac acc ggc acc aag | |||||
| GIOT-2 (T7) | Transcription repressor | F: cgc tgg aaa gaa acc cta tg | 221 | 57 | 1.25 | 0/29=0% |
| (AB021642)* | R: aaa cag gga agg ctt tag aac a | |||||
| PAIP (T6) | Polyadenylate binding protein interacting protein | F: taa gcc cca ggt ggt tgt ag | 228 | 61 | 2.5 | 0/30=0% |
| (AF013758)* | R: caa cca ttc agg gtc tct gc | |||||
| RIP3 (A6) | Receptor interacting serine/threonine kinase | F: tat ccc aga gtc agg cca ag | 221 | 59 | 2.5 | 0/30=0% |
| (NM_006871)* | R: ctg aag atg tgc ctg ctg tc |