Primers used for allele specific ARMS assays
| Mutation | Primers* | Size (bp) |
|---|---|---|
| *Accession numbers for the DHCR7 and HFE genes are AF110060 and Z92910, respectively. The coordinates for each primer are given relative to the genomic DNA sequence, with position 1 being the first base of the translation initiation codon. | ||
| IVS8-1G→C | (g.9279-9301) 5`-CACAACAGCCCTCAAGGTTGGTG-3` | 190 |
| (g.9468-9447) 5`-GTGGTACACCAAGTACAGTCCG-3` | ||
| T93M | (g.708-728) 5`-CAGTGTGACTGCCTGCATCCG-3` | 231 |
| (g.938-917) 5`-TAGAGCTGGGCGGCTTTCGTCA-3` | ||
| W151X | (g.3187-3208) 5`-CATACCACAGGGGCATCCTCAC-3` | 388 |
| (g.3574-3553) 5`-CAGAGCAGGTGCGTGAGGTGCT-3` | ||
| V326L | (g.9279-9301) 5`-CACAACAGCCCTCAAGGTTGGTG-3` | 202 |
| (g.9480-9460) 5`-CAGCTGCACGGGGTGGTTCAGA-3` | ||
| R404C | (g.9675-9694) 5`-GGGCTTCTGGGGCGTGCCCT-3` | 286 |
| (g.9964-9942) 5`-TGGCAGAACACGCTCTTGACAGC-3` | ||
| Internal control | (g.3335-3357) 5`-GTGTGTGGAGCCTCAACATCCTG-3` | 429 |
| (g.3763-3743) 5`-CACAAGACCTCAGACTTCCAG-3` | ||