Oligonucleotide sequences flanking each of the exons of the PGT, GATA2, and EGR2 genes. GATA2 has two alternatively used, non-coding, exons 1; we named these exon 1a and 1b. Exon 2 of the EGR2 gene was divided into eight amplicons (a–h) to facilitate PCR and subsequent sequencing. Amplification conditions are available upon request
| Forward 5`→3` | Reverse 5`→3` | Product size (bp) | |
|---|---|---|---|
| PGT | |||
| Exon 1 | GAGGGAGAGCGCGTTTCATC | CGGGTGCCCAGCCGAAGG | 351 |
| Exon 2 | GCTTGTCGTCCAGAAACTCACG | GGATCTTGTGGTCAGGGTGGTG | 240 |
| Exon 3 | TTCTCTGTGGGTGGGTGAGGACAG | CCATTCCTTACCCCTTGAGGC | 267 |
| Exon 4 | GTGGAGACCAGCAAGCACTCTC | GCTACATCAGGACCTCACCCATC | 310 |
| Exon 5 | CCTTTTCCAGCCATCTTATTTGCC | TTCTCTGTTCTGATTGGATGAGGG | 192 |
| Exon 6 | AAATCAGTAAACTCCCCCTGATC | TCCCTTTCTTCAACTCCATCAGG | 130 |
| Exon 7 | CGTGAACACTCACCCCTTTATTG | GTCACTGTGCCAGCCCACC | 149 |
| Exon 8 | GGGGTTCTGCCAGAGCTTGAC | GGGTGGCTGGAGTGCCTG | 268 |
| Exon 9 | TAGCCCACATGCAGTCCCAG | AGTTAAGGAAGCAGGAAGGAAGATG | 337 |
| Exon 10 | TGGTCTTGCTTTGTTTGATTCCC | GCCTATCCTGGAGCCGAGAAAC | 306 |
| Exon 11 | AATTTCCCCCTTTTTCCCTCC | AGAAGCCACGCCCCAGACTC | 238 |
| Exon 12 | CTGCTGATTTCCTGTCTGTCTTTTC | TTCTTGGGAAGAGGGGTCTTAGAC | 122 |
| Exon 13 | GCAGTTCCACCTGACCAGGG | TGGGACACACATACATGATGGG | 193 |
| Exon 14 | AGAGCCCCCTCCCTCACCAG | CAAGGTCCACTCTCTGGAGCAG | 201 |
| GATA2 | |||
| Exon1a | CGAGGCGCACTCTACCCCCA | CAGAGTCCCCTCAAAGCTAG | 207 |
| Exon1b | CAGGCGGCTGGACCTGGTAG | GCAAACGGACCACAGCGATTCG | 532 |
| Exon2 | CACCTCGTGGTGGGACTTTG | GATTCCTGCGGATCCTACATC | 430 |
| Exon3 | GAGTCGTGATCTCAATGTCTG | GAAACCAACACTGCCACCTC | 837 |
| Exon4 | TCCTGCCCAGGCTGTTGCAG | AAATCTGGCCCGAAAGAATCTG | 383 |
| Exon5 | GATTTAGCCCTCCTTGACTG | CAAGCTGGATCTTGTGGCTG | 314 |
| Exon6 | CTCAGCTTGACCTGCCTCTG | GTGTCGGCTTCGGGAAATGC | 460 |
| EGR2 | |||
| Exon1 | AAGTGTGGAGGGCAAAAGGA | ACGCGGCTTACCTCCGGC | 299 |
| Exon2a | TCCCCTTCCCCAGATGGCATG | TGACGCTGGATGAGGCTGTG | 248 |
| Exon2b | GCTACCCAGAAGGCATAATC | CGGAAGGAGGTGGTGGGTAG | 300 |
| Exon2c | CCCTTCTGCGTTCCTGTCAG | CAGGGTAAAGTTACGGATTG | 273 |
| Exon2d | ACCCAGGTGTCTTCCCAATG | GCAGGTGGTGTGGGTTATAG | 306 |
| Exon2e | CAATCCGTAACTTTACCCTG | GGATGTGCCGTGTCAGCTCG | 286 |
| Exon2f | CGAGCTGACACGGCACATCC | CTCCGCCAAGACTGCTGCTG | 326 |
| Exon2g | CGCAAGTACCCCAACAGACC | GGTGGTCACTGCGGCTGAAG | 186 |
| Exon2h | ATCGGTGCCAGCCCCCTCTA | TAGGTGGAAAGGGGGCAGTG | 287 |