Expression of NAG in transfected COS-7 cells
| Transfected vector | α-N-acetylglucosaminidase activity (nmol/h/mg protein) | Total (% of wild type) |
|---|---|---|
| The NAG activities in COS-7 cells are the mean of two independent experiments, with a range of less than 5%. The endogenous NAG activity in COS-7 cells was subtracted before the percentage of wild type was calculated. | ||
| pcDNA3 | 3.80 | 0.0 |
| pcDNA3-NAG | 54.25 | 100.0 |
| pcDNA3-NAG/R130C | 3.80 | 0.0 |
| pcDNA3-NAG/I154R | 4.00 | 0.4 |
| pcDNA3-NAG/660delC | 3.80 | 0.0 |
| pcDNA3-NAG/Y309C | 5.41 | 3.2 |
| pcDNA3-NAG/G412E | 3.85 | 0.1 |
| pcDNA3-NAG/R565W | 5.77 | 3.9 |
| pcDNA3-NAG/R626X | 3.80 | 0.0 |