Fragment length of PCR products for six RFLPs in the fibrinogen gene
RFLP | Gene | Location | Amplicon size (bp) | Allele designation | Digested fragment length (bp) |
TaqI | α gene | 3′ end | 900 | T2 | TaqI(+): 100, 800 |
T1 | TaqI(−): 900 | ||||
T312A | α gene | A/G (codon 312) | 584 | T312 | RsaI(+): 78, 39,170, 136, 56, 44,39 |
A312 | RsaI(−): 117,170, 136, 56, 44,39 | ||||
−455G/A | β gene | G/A−455 (promoter) | 1301 | G−455 | HaeIII(+): 272, 194, 835 |
A−455 | HaeIII(−): 466, 835 | ||||
−148C/T | β gene | C/T−148 (promoter) | 1301 | C−148 | HindIII(+): 575, 383, 343 |
T−148 | HindIII(−): 958, 343 | ||||
+1689T/G | β gene | T/G+1689 (intron 1) | 710 | G+1689 | AvaII(+): 250, 60, 400 |
T+1689 | AvaII(−): 310, 400 | ||||
BclI | β gene | 3′ end | 2500 | B2 | BclI(+): 1400, 1100 |
B1 | BclI(−): 2500 |
Underlined fragments are constant bands in both alleles from each polymorphism as an internal control of enzyme activity.