Table 1

Fragment length of PCR products for six RFLPs in the fibrinogen gene

RFLP Gene Location Amplicon size (bp) Allele designation Digested fragment length (bp)
TaqIα gene3′ end900T2 TaqI(+): 100, 800
T1 TaqI(−): 900
T312Aα geneA/G (codon 312)584T312 RsaI(+): 78, 39,170, 136, 56, 44,39
A312 RsaI(−): 117,170, 136, 56, 44,39
−455G/Aβ geneG/A−455 (promoter)1301G−455 HaeIII(+): 272, 194, 835
A−455 HaeIII(−): 466, 835
−148C/Tβ geneC/T−148 (promoter)1301C−148 HindIII(+): 575, 383, 343
T−148 HindIII(−): 958, 343
+1689T/Gβ geneT/G+1689 (intron 1)710G+1689 AvaII(+): 250, 60, 400
T+1689 AvaII(−): 310, 400
BclIβ gene3′ end2500B2 BclI(+): 1400, 1100
B1 BclI(−): 2500
  • Underlined fragments are constant bands in both alleles from each polymorphism as an internal control of enzyme activity.