Table 1

Primer sets used in the amplification of STRP loci genotyped in the FJHN/MCKD2 candidate region

Primer (5′→3′)
LocusForwardReverseSTRP* size (bp)STRP typeRelative position†
*STRP size indicates the region that the PCR amplified band will be in.
†Relative position refers to the locus location on the BAC contig alignment sequence.
‡This position determined using the Human Genome Project data from June 2002.
Amplifications performed using standard Amplitaq Gold Conditions
D16S499TCTCACAGTTCTGGAGGCTGGAAGGGTGGACCCTAATTGCATAGGATTG210CA repeat 238 700
D16S501TGTCCTCTAGGGGAAGAGATGTCTAGGTCAGGGACCTAGTAACTACTC260CA repeat 305 100
481E9(16)CCAGAGCCCTACAGGAGTGTACTGCAAGACCAGGGGATCACAGTAACT320Di 362 700
449G13(16)CAGCCTGGGCAACAGAGACTCAGGCGCTAAATTCAGAGCAAATAG300CA repeat1 784 000
419L9(16)GCTGTAATGGTGCTGTGTAAATCTAAGAATCCTCCAGACTTCATACAC218CA repeat1 983 000
626G11(16)ATCAGCTTAGCAGACATCTCTTCCCTTGTAGTCCCAGCTACTCAGTGG292CA repeat2 019 000
2349B8(16)-2CACGAGAATCCCTTGAACCTGTGGCTCTCCACTCAGAGATTC214Penta2 050 000
2349B8(16)-1CTGTGGCTGGCTTGTTTCACTCAGTTGGGTGGAGGCAATCCAAGTGTC201CA repeat2 133 000
363E6(16)TGTGTTATTGGTGAAATGCACATAGGTGGCTCATGCCTGTAATTTGAG355Di2 250 000
D16S3041Applied Biosystem Linkage Mapping Set, Panel 73270CA repeat2 310 000
D16S490TGACAGGCACATAGATTATTATGCCGTACCCGGCTGATTATTTTAGAT357Tetra2 390 000
D16S3036AGATAGGGGTCTAGTTTCATTATCACAAAGCTGGACATATCACACTAC310CA repeat2 450 000
2380F24(16)AGGCTGGTCTCGAACTCCTGACCTGGGACTACAGGTGTGTGAATTTGA272Di2 730 000
D16S3046Applied Biosystem Linkage Mapping Set, Panel 22110CA repeat3 650 000
D16S3045AGGACGGCTGAATGTCTGTCATCATTGGGGAGTCCCTAAATGACTTTA180CA repeat3 790 000
14O15(16)GGCAGAAATGGCACATCTTAACTACAGCCTGGGTGACAGAGTGAGACT234CA repeat5 040 000
D16S403Applied Biosystem Linkage Mapping Set, Panel 73150CA repeat5 820 000
D16S412ACCCAGTAGAGACCCATCTTACTCACCCAGTAGAGACCCATCTTACTC180CA repeat5 952 000‡