Primers used to amplify all exons of the MSH6 gene. In order to facilitate subsequent direct sequencing, 5′ primers were tailed with M13 forward (TGTAAAACGACGGCCAGT) and M13 reverse (CAGGAAACAGCTATGACC) sequences
| MSH6 | Primer sequences | |
| Exon 1 | Fw: TGTAAAACGACGGCCAGTTCCGTCCGACAGAACGGTTG | |
| Rv: CAGGAAACAGCTATGACCCCCCAAATGCTCCAGACTCG | ||
| Exon 2 | Fw: TGTAAAACGACGGCCAGTGCCAGAAGACTTGGAATTGTTTATTTG | |
| Rv: CAGGAAACAGCTATGACCACACAAACACACACACATGGCAGTAG | ||
| Exon 3 | Fw: TGTAAAACGACGGCCAGTCGTGAGCCTCTGCACCCGGCCC | |
| Rv: CAGGAAACAGCTATGACCCCCCATCACCCTAACATAAA | ||
| Exon 4A | Fw: TGTAAAACGACGGCCAGTGTCTTACATTATGGTTTTCC | |
| Rv: CAGGAAACAGCTATGACCCCACATCAGAGCCACCAATG | ||
| Exon 4B | Fw: TGTAAAACGACGGCCAGTCGAAGGGTCATATCAGATTC | |
| Rv: CAGGAAACAGCTATGACCATACCAAACAGTAGGGCGAC | ||
| Exon 4C | Fw: TGTAAAACGACGGCCAGTCGTTAGTGGAGGTGGTGATG | |
| Rv: CAGGAAACAGCTATGACCATGAATACCAGCCCCAGTTC | ||
| Exon 4D | Fw: TGTAAAACGACGGCCAGTCTGTACCACATGGATGCTCT | |
| Rv: CAGGAAACAGCTATGACCCTTCCTCTTTTTCTTTGAGG | ||
| Exon 4E | Fw: TGTAAAACGACGGCCAGTAAAGTAGCACGAGTGGAACAGACTGAG | |
| Rv: CAGGAAACAGCTATGACCAACATCACCCCAATGCCATCAC | ||
| Exon 4F | Fw: TGTAAAACGACGGCCAGTCTGTTCTCTTCAGGAAGGTC | |
| Rv: CAGGAAACAGCTATGACCAGCCATTGCTTTAGGAGCCG | ||
| Exon 4G | Fw: TGTAAAACGACGGCCAGTACTTGCCATACTCCTTTTGG | |
| Rv: CAGGAAACAGCTATGACCCCTGCTTTGGGAGTAATAAG | ||
| Exon 4H | Fw: TGTAAAACGACGGCCAGTGAAAAGGCTCGAAAGACTGG | |
| Rv: CAGGAAACAGCTATGACCCCAAAGGGCTACTAAGATAAAAGCGAG | ||
| Exon 5 | Fw: TGTAAAACGACGGCCAGTGGGGAGATCGTTGGACTGTAATTG | |
| Rv: CAGGAAACAGCTATGACCTTGCTTCCTATTAAGTCACTGGCTG | ||
| Exon 6 | Fw: TGTAAAACGACGGCCAGTGTTTATGAAACTGTTACTACC | |
| Rv: CAGGAAACAGCTATGACCGCAAATATCTTTTATCACAT | ||
| Exon 7 | Fw: TGTAAAACGACGGCCAGTGCCAATAATTGCATAGTCTCTTAATG | |
| Rv: CAGGAAACAGCTATGACCGCCACAATGGTGAGTGCGTG | ||
| Exon 8 | Fw: TGTAAAACGACGGCCAGTCCTTTTTTGTTTTAATTCCT | |
| Rv: CAGGAAACAGCTATGACCCAACAGAAGTGCCCTCTCAA | ||
| Exon 9 | Fw: TGTAAAACGACGGCCAGTGATTATTCTCAAAATGTTGCTGTGCG | |
| Rv: CAGGAAACAGCTATGACCTTCACTAGCCAGGCAAACTTCCC | ||
| Exon 10 | Fw: TGTAAAACGACGGCCAGTATTTTAAGGGAAGTTTGCC | |
| Rv: CAGGAAACAGCTATGACCGTTTATTAGATCATAATGTT | ||