STRP markers for human cathespin C gene interval
| STRP | Location1-150 | Allele size (bp) | Primer | Allele frequency | Tan (°C) | ||||
| CTSC-01 | 8505–8811 | 306–322 | F 5′-TAGGGCAAAATGTGTCTGAAGAGT-3′1-151 | 306(.10); 308(.20); 310(.34); | 58 | ||||
| R 5′-TACCCATGAGATGGCTTTCATCAG-3′ | 312(.04); 314(.08); 318(.17); | ||||||||
| 320(.04); 322(.03)1-152 | |||||||||
| CTSC-5′ | 57835–58180 | 332–364 | F 5′-GCAGCACAGGACGGCTTTGAATAC-3′1-151 | 332(.01); 334(.02); 336(.07); | 58 | ||||
| R 5′-GATGGTGAAACCCTGTCTCTACTA-3′ | 338(.01); 340(.02); 342(.04); | ||||||||
| 344(.01); 346(.10); 348(.08); | |||||||||
| 350(.07); 352(.07); 354(.07); | |||||||||
| 356(.23); 358(.01); 360(.15); | |||||||||
| 362(.01); 364(.03)1-152 | |||||||||
| CTSC-int3 | 95935–96181 | 239–251 | F 5′-TATGGAAGAGTGCTCACAACTATA-3′1-151 | 239(.02); 241(.15); 243(.06); | 60 | ||||
| R 5′-TCTCATGCCACCATTCAGAGGTAA-3′ | 245(.10); 247(.62); 249(.03); | ||||||||
| 251(.02)1-152 | |||||||||
| CTSC-3′ | 114813–115158 | 347–381 | F 5′-CTAAACTCAGGCCCTTCATCATTC-3′ | 347(.43); 353(.02); 355(.01); | 58 | ||||
| R 5′-ACCCCTCATAAGCCCTTCTCTATC-3′1-151 | 361(.02); 363(.24); 365(.06); | ||||||||
| 367(.02); 369(.05); 371(.04); | |||||||||
| 373(.10); 381(.01)1-152 | |||||||||
| CTSC-02 | 165674–165835 | 148–168 | F 5′-TAGCAGAGAAGAAGCAAGATGTGT-3′ | 148(.04); 150(.03); 152(.04); | 58 | ||||
| R 5′-AAGCTGGACAAGGAGACTGTATAG-3′1-151 | 154(.28); 156(.05); 158(.06); | ||||||||
| 160(.25); 162(.07); 164(.15); | |||||||||
| 166(.02); 168(.01)1-152 | |||||||||
| CTSC-03 | 171820–172020 | 193–213 | F 5′-CTCCCTATCCATTCTCAGTATCTA-3′1-151 | 193(.05); 195(.17); 197(.22); | 58 | ||||
| R 5′-CCAGAGTCACAGCAAATCTCAGAG-3′ | 199(.09); 201(.18); 203(.11); | ||||||||
| 205(.12); 207(.02); 209(.03); | |||||||||
| 213(.01)1-152 | |||||||||
| D11S1887M | 93 | 237–257 | F 5′-CAGGCTTCAAAGGCTTAATGTGAA-3′ | 237(.33); 239(.15); 241(.11); | 58 | ||||
| R 5′-CCTACCTGCAACATGAGATGAGTG-3′1-151 | 243(.11); 245(.07); 249(.04); | ||||||||
| 251(.04); 253(.11); 257(.04)1-153 | |||||||||
| D11S1780M | 94.6 | 263–289 | F 5′-TTCAGGGATCTGCAGCAATTACTT-3′ | 263(.04); 271(.28); 273(.14); | 58 | ||||
| R 5′-TCTGAGATTACAGGCGTGAGTCAC-3′1-151 | 275(.04); 281(.32); 287(.04); | ||||||||
| 289(.14)¶ | |||||||||
| D11S1367M | 95–96 | 229–254 | F 5′-TTAAATCTTGGTAGCCAGAACTAT-3′1-151 | 221(.03); 229(.20); 233(.26); | 58 | ||||
| R 5′-ATTGTGCTAGGCTCTCTAAATACA-3′ | 237(.10); 241(.17); 245(0.10); | ||||||||
| 249(.07); 253(.07)** | |||||||||
| D11S1358M | 96.3 | 273–283 | F 5′-AGCTCAAGTGCCTGTCAACAGATG-3′1-151 | 273(.21); 275(.04); 277(.13); | 58 | ||||
| R 5′-TGCGATTCTCCTGGCTCTTAGAAC-3′ | 279(.33); 281(.25); 283(.04)†† | ||||||||
↵1-150 The location of the CTSC specific marker is based on the BAC contig spanning the cathepsin C gene (fig 2). For the modified (M) D11S markers, the cM location is the distance from the telomeric end of the short arm on chromosome 11 based on GB4 map (www.ncbi.nlm.nih.gov/genemap99) and the physical map of 30 000 human genes.20
↵1-151 Location of the fluorescent label for each primer set.
↵1-152 Based on 112 chromosomes of Turkish controls.
↵1-153 ¶ ** ††Based on the PLS patients in this study. The founder effects of each haplotype were taken in consideration with these patients based on 25, 22, 30, and 24 chromosomes, respectively. Tan: annealing temperature for PCR.